Modification of senescence in ryegrass transformed with IPT under the control of a monocot senescence-enhanced promoter

Q. Li, Paul R. H. Robson, Andy J. E. Bettany, Iain S. Donnison, Howard Thomas, Ian M. Scott

Research output: Contribution to journalArticlepeer-review

Abstract

We report here the genetic modification of ryegrass senescence. Embryogenic cell suspensions of Lolium multiflorum were transformed by microprojectile bombardment with plasmid constructs containing 1.98 kb of the 5 flanking sequence of SEE1 (a maize cysteine protease gene showing enhanced expression during senescence) fused either to the Agrobacterium tumefaciens cytokinin biosynthesis gene IPT (designated PSEE1::IPT) or to the -glucuronidase reporter gene UIDA (PSEE1::UIDA). Plants were regenerated under selection for the HPH hygromycin resistance gene in the vector. PSEE1::UIDA transformants confirmed that the SEE1 flanking sequence functioned as a senescence-enhanced promoter in ryegrass. The IPT transgene was detected in 28 regenerants (PSEE1::IPT) from five independent transformation events. PSEE1::IPT leaves displayed a stay-green phenotype. Some PSEE1::IPT lines developed spontaneous lesions.
Original languageEnglish
Pages (from-to)816-821
Number of pages6
JournalPlant Cell Reports
Volume22
Issue number11
Early online date13 Feb 2004
DOIs
Publication statusPublished - Jun 2004

Keywords

  • cysteine protease
  • IPT
  • promoter
  • transgenic ryegrass
  • senescence

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