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Abstract
Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging parasite of livestock with many aspects of its underpinning biology yet to be resolved. This research is the first in-depth investigation of EVs released by adult C. daubneyi. EVs were successfully isolated using both differential centrifugation and size exclusion chromatography (SEC), and morphologically characterized though transmission electron microscopy (TEM). EV protein components were characterized using a GeLC approach allowing the elucidation of comprehensive proteomic profiles for both their soluble protein cargo and surface membrane bound proteins yielding a total of 378 soluble proteins identified. Notably, EVs contained Sigma-class GST and cathepsin L and B proteases, which have previously been described in immune modulation and successful establishment of parasitic flatworm infections. SEC purified C. daubneyi EVs were observed to modulate rumen bacterial populations by likely increasing microbial species diversity via antimicrobial activity. This data indicates EVs released from adult C. daubneyi have a role in establishment within the rumen through the regulation of microbial populations offering new routes to control rumen fluke infection and to develop molecular strategies to improve rumen efficiency.
Original language | English |
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Article number | 661830 |
Number of pages | 12 |
Journal | Frontiers in Cellular and Infection Microbiology |
Volume | 11 |
DOIs | |
Publication status | Published - 20 Apr 2021 |
Keywords
- Calicophoron daubneyi
- Cellular and Infection Microbiology
- extracellular vesicle
- mass spectrometry
- proteomics
- rumen microbiome
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- 1 Finished
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Improving diagnostic of liver and rumen fluke livestock parasites utilising exosome-like
Morphew, R. (PI)
01 Apr 2015 → 31 Mar 2018
Project: Externally funded research