Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation

Yan Zhang, Zhi-Xia Li, Xiu-Dao Yu, Jia Fan, John A. Pickett, Huw D. Jones, Jing-Jiang Zhou, Michael A. Birkett, John Caulfield, Johnathan A. Napier, Guang-Yao Zhao, Xian-Guo Cheng, Yi Shi, Toby J. A. Bruce, Lan-Qin Xia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (SciVal)

Abstract

Aphids are important pests of wheat (Triticum aestivum) that affect crop production globally. Herbivore-induced emission of sesquiterpenes can repel pests, and farnesyl pyrophosphate synthase (FPS) is a key enzyme involved in sesquiterpene biosynthesis. However, fps orthologues in wheat and their functional roles in sesquiterpene synthesis and defence against aphid infestation are unknown. Here, two fps isoforms, Tafps1 and Tafps2, were identified in wheat. Quantitative real-time polymerase chain reaction (qRT-PCR) and invitro catalytic activity analyses were conducted to investigate expression patterns and activity. Heterologous expression of these isoforms in Arabidopsis thaliana, virus-induced gene silencing (VIGS) in wheat and aphid behavioural assays were performed to understand the functional roles of these two isoforms. We demonstrated that Tafps1 and Tafps2 played different roles in induced responses to aphid infestation and in sesquiterpene synthesis. Heterologous expression in A.thaliana resulted in repulsion of the peach aphid (Myzus persicae). Wheat plants with these two isoforms transiently silenced were significantly attractive to grain aphid (Sitobion avenae). Our results provide new insights into induced defence against aphid herbivory in wheat, in particular, the different roles of the two Tafps isoforms in both sesquiterpene biosynthesis and defence against aphid infestation.

Original languageEnglish
Pages (from-to)1101-1115
Number of pages15
JournalNew Phytologist
Volume206
Issue number3
Early online date30 Jan 2015
DOIs
Publication statusPublished - 10 Apr 2015
Externally publishedYes

Keywords

  • aphid
  • farnesyl pyrophosphate synthase gene (fps)
  • grain aphid (Sitobion avenae)
  • inducible defence
  • peach aphid (Myzus persicae)
  • sesquiterpenes
  • virus-induced gene silencing (VIGS)
  • wheat (Triticum aestivum)
  • DIPHOSPHATE SYNTHASE
  • ARABIDOPSIS-THALIANA
  • ARTEMISIA-ANNUA
  • COORDINATE REGULATION
  • BIOSYNTHETIC-PATHWAY
  • TRANSGENIC PLANTS
  • DRAFT GENOME
  • CLONING
  • EXPRESSION
  • CDNA

Fingerprint

Dive into the research topics of 'Molecular characterization of two isoforms of a farnesyl pyrophosphate synthase gene in wheat and their roles in sesquiterpene synthesis and inducible defence against aphid infestation'. Together they form a unique fingerprint.

Cite this