Molecular diversity of the rumen microbiome of Norwegian reindeer on natural summer pasture

Monica A. Sundset, Joan Elizabeth Edwards, Yan Fen Cheng, Roberto S. Senosiain, Maria N. Fraile, Korinne S. Northwood, Kirsti E. Præsteng, Trine Glad, Svein D. Mathiesen, André-Denis G. Wright

Research output: Contribution to journalArticlepeer-review

80 Citations (SciVal)

Abstract

The molecular diversity of the rumen microbiome was investigated in five semi-domesticated adult female Norwegian reindeer (Rangifer tarandus tarandus) grazing on natural summer pastures on the coast of northern Norway (71.00° N, 25.30° E). Mean population densities (numbers per gram wet weight) of methanogenic archaea, rumen bacteria and ciliate protozoa, estimated using quantitative real-time polymerase chain reaction (PCR), were 3.17 × 109, 5.17 × 1011 and 4.02 × 107, respectively. Molecular diversity of rumen methanogens was revealed using a 16S rRNA gene library (54 clones) constructed using pooled PCR products from the whole rumen contents of the five individual reindeer. Based upon a similarity criterion of
Original languageEnglish
Pages (from-to)335-348
Number of pages14
JournalMicrobial Ecology
Volume57
Issue number2
DOIs
Publication statusPublished - 01 Feb 2009

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