Molecular diversity of the rumen microbiome of Norwegian reindeer on natural summer pasture

Monica A. Sundset; Joan Elizabeth Edwards; Yan Fen Cheng; Roberto S. Senosiain; Maria N. Fraile; Korinne S. Northwood; Kirsti E. Præsteng; Trine Glad; Svein D. Mathiesen; André-Denis G. Wright

doi:10.1007/s00248-008-9414-7

Molecular diversity of the rumen microbiome of Norwegian reindeer on natural summer pasture

Monica A. Sundset, Joan Elizabeth Edwards, Yan Fen Cheng, Roberto S. Senosiain, Maria N. Fraile, Korinne S. Northwood, Kirsti E. Præsteng, Trine Glad, Svein D. Mathiesen, André-Denis G. Wright

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

The molecular diversity of the rumen microbiome was investigated in five semi-domesticated adult female Norwegian reindeer (Rangifer tarandus tarandus) grazing on natural summer pastures on the coast of northern Norway (71.00° N, 25.30° E). Mean population densities (numbers per gram wet weight) of methanogenic archaea, rumen bacteria and ciliate protozoa, estimated using quantitative real-time polymerase chain reaction (PCR), were 3.17 × 109, 5.17 × 1011 and 4.02 × 107, respectively. Molecular diversity of rumen methanogens was revealed using a 16S rRNA gene library (54 clones) constructed using pooled PCR products from the whole rumen contents of the five individual reindeer. Based upon a similarity criterion of

Original language	English
Pages (from-to)	335-348
Number of pages	14
Journal	Microbial Ecology
Volume	57
Issue number	2
DOIs	https://doi.org/10.1007/s00248-008-9414-7
Publication status	Published - 01 Feb 2009

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10.1007/s00248-008-9414-7

http://hdl.handle.net/2160/4561

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Plant stress responses in the rumen
Kingston-Smith, A. (PI)
Biotechnology and Biological Sciences Research Council
01 Apr 2008 → 31 Mar 2012
Project: Externally funded research

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title = "Molecular diversity of the rumen microbiome of Norwegian reindeer on natural summer pasture",

abstract = "The molecular diversity of the rumen microbiome was investigated in five semi-domesticated adult female Norwegian reindeer (Rangifer tarandus tarandus) grazing on natural summer pastures on the coast of northern Norway (71.00° N, 25.30° E). Mean population densities (numbers per gram wet weight) of methanogenic archaea, rumen bacteria and ciliate protozoa, estimated using quantitative real-time polymerase chain reaction (PCR), were 3.17 × 109, 5.17 × 1011 and 4.02 × 107, respectively. Molecular diversity of rumen methanogens was revealed using a 16S rRNA gene library (54 clones) constructed using pooled PCR products from the whole rumen contents of the five individual reindeer. Based upon a similarity criterion of ",

author = "Sundset, {Monica A.} and Edwards, {Joan Elizabeth} and Cheng, {Yan Fen} and Senosiain, {Roberto S.} and Fraile, {Maria N.} and Northwood, {Korinne S.} and Pr{\ae}steng, {Kirsti E.} and Trine Glad and Mathiesen, {Svein D.} and Wright, {Andr{\'e}-Denis G.}",

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AU - Northwood, Korinne S.

AU - Præsteng, Kirsti E.

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AU - Mathiesen, Svein D.

AU - Wright, André-Denis G.

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Molecular diversity of the rumen microbiome of Norwegian reindeer on natural summer pasture

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Plant stress responses in the rumen

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