Molecular phylogenetics of tsetse flies (Diptera: Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the palpalis group

N. A. Dyer, Scott Paul Lawton, S. Ravel, K. S. Choi, M. J. Lehane, A. S. Robinson, L. M. Okedi, M. J. R. Hall, P. Solano, M. J. Donnelly

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Abstract

Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In particular, cytochrome oxidase 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented.
Original languageEnglish
Pages (from-to)227-239
Number of pages13
JournalMolecular Phylogenetics and Evolution
Volume49
Issue number1
Early online date22 Jul 2008
DOIs
Publication statusPublished - 01 Oct 2008

Keywords

  • Tsetse
  • Glossina
  • Diagnostic PCR
  • Palpalis
  • Cryptic species

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