Molecular profiling of bacterial species in the rabbit caecum

Leticia Abecia; Manuel Fondevila; Joaquim Balcells; Joan E. Edwards; C. James Newbold; Neil R. McEwan

doi:10.1016/j.femsle.2005.01.028

Molecular profiling of bacterial species in the rabbit caecum

Leticia Abecia, Manuel Fondevila, Joaquim Balcells, Joan E. Edwards, C. James Newbold, Neil R. McEwan

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

The diversity of bacteria present in the caecum of the rabbit was investigated. Partial bacterial 16S rRNA genes from a digested sample collected from the caecum of an adult rabbit were amplified by PCR. Sequence analysis of the amplified fragments indicated highest similarity was to bacterial sequences previously described from other gut environments. However, only one sequence showed significant identity (97% threshold) to any previously described bacterial 16S rRNA genes. Furthermore, most of the sequences clustered together in groups lacking representatives from sequences already described, suggesting that the rabbit caecal flora contains organisms not previously described.

Original language	English
Pages (from-to)	111-115
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	244
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.01.028
Publication status	Published - Mar 2005

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title = "Molecular profiling of bacterial species in the rabbit caecum",

abstract = "The diversity of bacteria present in the caecum of the rabbit was investigated. Partial bacterial 16S rRNA genes from a digested sample collected from the caecum of an adult rabbit were amplified by PCR. Sequence analysis of the amplified fragments indicated highest similarity was to bacterial sequences previously described from other gut environments. However, only one sequence showed significant identity (97% threshold) to any previously described bacterial 16S rRNA genes. Furthermore, most of the sequences clustered together in groups lacking representatives from sequences already described, suggesting that the rabbit caecal flora contains organisms not previously described.",

author = "Leticia Abecia and Manuel Fondevila and Joaquim Balcells and Edwards, {Joan E.} and Newbold, {C. James} and McEwan, {Neil R.}",

note = "Abecia, L., Fondevila, M., Balcells, J., Edwards, J. E., Newbold, C. J., McEwan, N. R. (2005). Molecular profiling of bacterial species in the rabbit caecum. FEMS Microbiology Letters, 244 (1), 111-115. RAE2008",

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AU - Abecia, Leticia

AU - Fondevila, Manuel

AU - Balcells, Joaquim

AU - Edwards, Joan E.

AU - Newbold, C. James

AU - McEwan, Neil R.

N1 - Abecia, L., Fondevila, M., Balcells, J., Edwards, J. E., Newbold, C. J., McEwan, N. R. (2005). Molecular profiling of bacterial species in the rabbit caecum. FEMS Microbiology Letters, 244 (1), 111-115. RAE2008

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N2 - The diversity of bacteria present in the caecum of the rabbit was investigated. Partial bacterial 16S rRNA genes from a digested sample collected from the caecum of an adult rabbit were amplified by PCR. Sequence analysis of the amplified fragments indicated highest similarity was to bacterial sequences previously described from other gut environments. However, only one sequence showed significant identity (97% threshold) to any previously described bacterial 16S rRNA genes. Furthermore, most of the sequences clustered together in groups lacking representatives from sequences already described, suggesting that the rabbit caecal flora contains organisms not previously described.

AB - The diversity of bacteria present in the caecum of the rabbit was investigated. Partial bacterial 16S rRNA genes from a digested sample collected from the caecum of an adult rabbit were amplified by PCR. Sequence analysis of the amplified fragments indicated highest similarity was to bacterial sequences previously described from other gut environments. However, only one sequence showed significant identity (97% threshold) to any previously described bacterial 16S rRNA genes. Furthermore, most of the sequences clustered together in groups lacking representatives from sequences already described, suggesting that the rabbit caecal flora contains organisms not previously described.

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DO - 10.1016/j.femsle.2005.01.028

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VL - 244

SP - 111

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

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Molecular profiling of bacterial species in the rabbit caecum

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