Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Soluble (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion

David Neil Bryant, Elliot Firth, Naheed Nazly Kaderbhai, Stephen Taylor, Stephen Michael Morris, Daniel Logan, Naroa Garcia, Andrew Ellis, Steven M. Martin, Joseph Anthony Gallagher

Research output: Contribution to journalArticlepeer-review

9 Citations (SciVal)

Abstract

Dielectric spectroscopy (DS) has been used to monitor the simultaneous saccharification and fermentation of lignocellulosic biomass by measuring its dielectric state. However, it is unknown whether following steam explosion (SE) pre-treatment, lignocellulose would still maintain a dielectric state, and, if maintained, whether the dissipation during enzymatic hydrolysis could be monitored. Distillers Dried Grains with Solubles (DDGS), pre-treated by SE, was found to have a capacitance (C=580kHz) of approximately 24pFcm(-1). Following addition of full-strength cellulolytic cocktail A (CC-A; R(2)=0.97) and 1/3 strength cocktail B (CC-B; R(2)=0.96), a natural logarithmic decay in capacitance was determined. Furthermore, the DS biomass probes quantified the initial linear rate of dissipation in capacitance during hydrolysis. The rate of CC-B was 34% that of CC-A. These data extend scope and utility of DS biomass probes for monitoring the enzymatic hydrolysis of SE-pre-treated lignocellulosic substrates in real-time.
Original languageEnglish
Pages (from-to)765-768
JournalBioresource Technology
Volume128
Early online date15 Sept 2012
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Steam explosion
  • Lignocellulose
  • Dielectric spectroscopy
  • Distillers Dried Grains with Solubles
  • DDGS

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