Murine T cell-stimulatory peptides from the 19-kDa antigen of Mycobacterium tuberculosis: Epitope-restricted homology with the 28-kDa protein of Mycobacterium leprae

D. P. Harris, H. M. Vordermeier, E. Roman, R. Lathigra, S. J. Brett, C. Moreno, J. Ivanyi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Fifteen overlapping synthetic peptides, spanning the entire amino acid sequence of the Mycobacterium tuberculosis 19-kDa protein, were used to identify epitopes recognized by murine T cells. Five of the 15 peptides tested were able to elicit in vitro lymph node T cell proliferative responses in C57BL/10 mice primed by footpad inoculation with homologous peptide. Analysis in congenic strains of mice revealed H-2 restriction in the response to four peptides. However, one peptide, 19.7 (residues 61 to 80), induced T cell responses in all four haplotypes tested. This peptide was also unique in being able to stimulate lymph node cells from C57BL/10 mice immunized with recombinant 19-kDa protein, killed M. tuberculosis, or live bacillus Calmette Guerin infection. T cell lines specific for peptide 19.7 were of the CD4 phenotype. Significantly, sequence analysis revealed that residues 61 to 80 of the 19-kDa protein exhibited considerable homology with a single 20-amino acid sequence (residues 120 to 140), but not with any other region of the 28-kDa protein expressed in Mycobacterium leprae. This finding is the first evidence of epitope-restricted homology between otherwise structurally unrelated microbial Ag.

Original languageEnglish
Pages (from-to)2706-2712
Number of pages7
JournalJournal of Immunology
Volume147
Issue number8
Publication statusPublished - 15 Oct 1991

Keywords

  • Amino Acid Sequence
  • Animals
  • Antigens, Bacterial/immunology
  • Epitopes/analysis
  • H-2 Antigens/genetics
  • Haplotypes
  • Immunization
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mycobacterium bovis/immunology
  • Mycobacterium leprae/immunology
  • Mycobacterium tuberculosis/immunology
  • Peptide Fragments/immunology
  • Structure-Activity Relationship
  • T-Lymphocytes/immunology

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