Mutagenesis in Lotus japonicus

K. Judith Webb, Mark P. Robbins, Trevor Wang, A. J. Marquez, A. Meignez, M. Parniske

Research output: Chapter in Book/Report/Conference proceedingChapter


Lotus Japonicus, A General Introduction: Lotus japonicus as a model system.- Lotus-related species and their agronomic importance.-Drought and saline stress.-Symbiotic Processes: Methods for studying nodule development and function.-A procedure for in vitro nodulation studies.- Arbuscular mycorrhiza.-Cell And Molecular Biology Protocols: Concurrent visualization of gusA and lacZ reporter gene expression.- Embedding root and nodule tissue in plastic (BMM).- RNA isolation using phase extraction and LiCl precipitation.-RNA isolation using CsCl gradients.-96-well DNA isolation method.-Functional Genomics: Genome structural analysis.-Transcriptome analysis using cDNA arrays.-Lotus japonicus expression database.-Procedures for mass spectrometric proteome analysis.-Isolation of peribacteroid membranes for proteome analysis.-Metabolome analysis using GC-MS.-Mutants, Gene-Tagging, And Genetic Analysis: Mutagenesis.-A mutant catalogue of Lotus japonicus.- Tilling.-Ds gene tagging.-Mapping and map-based cloning.-An in silico strategy using comparative sequence analysis.-Information transfer: mapping and cloning in other legumes.-Transformation And Reverse Genetic Analysis: Agrobacterium-mediated in vitro transformation.- Induction of hairy roots for symbiotic gene expression studies.-Transformation-regeneration procedure.-Agrobacterium rhizogenes pRi TL-DNA integration system.-Vectors for reverse genetics and expression analysis.-Primary And Secondary Metabolism: Nitrate assimilation;Influence of nitrogen supply.- Nitrate and ammonium assimilatory enzymes.-Transgenic plants affected in nitrate assimilation.-Secondary metabolite profiling.-Phenolic compounds:extraction and analysis.-Elicitation of isoflavan phytoalexins.-Lotus Japonicus Literature:An update of work published on Lotus japonicus.- Index
Original languageEnglish
Title of host publicationLotus Handbook
EditorsA. J. Marquez
PublisherSpringer Nature
Number of pages10
ISBN (Print)9.781402037344E12
Publication statusPublished - 2005


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