Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice

Elihu Aranday Cortes, Daryan Kaveh, Javier Nunez-Garcia, Philip J. Hogarth, Martin H. Vordermeier*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (SciVal)

Abstract

Background: In the present study, we applied microarray technology to define biosignatures by microarray transcriptome analysis in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, these biosignatures should be detectable without in vitro antigenic challenge. Principal Findings: After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytokine profile was found that correlated with vaccine-induced protective immunity following infection with virulent M. bovis in the lung as well as additional genes that were up-regulated in the spleens of vaccinated animals post-infection related to neutrophil biology and inflammation. Conclusions: This study has therefore prioritized both biomarkers predicting vaccination success before challenge and bio-signatures that are potentially associated with protective immune responses that will be useful to evaluate future vaccine candidates.

Original languageEnglish
Article numbere11319
JournalPLoS One
Volume5
Issue number6
Early online date28 Jun 2010
DOIs
Publication statusPublished - 12 Aug 2010

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