TY - JOUR
T1 - Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice
AU - Aranday Cortes, Elihu
AU - Kaveh, Daryan
AU - Nunez-Garcia, Javier
AU - Hogarth, Philip J.
AU - Vordermeier, Martin H.
PY - 2010/8/12
Y1 - 2010/8/12
N2 - Background: In the present study, we applied microarray technology to define biosignatures by microarray transcriptome analysis in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, these biosignatures should be detectable without in vitro antigenic challenge. Principal Findings: After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytokine profile was found that correlated with vaccine-induced protective immunity following infection with virulent M. bovis in the lung as well as additional genes that were up-regulated in the spleens of vaccinated animals post-infection related to neutrophil biology and inflammation. Conclusions: This study has therefore prioritized both biomarkers predicting vaccination success before challenge and bio-signatures that are potentially associated with protective immune responses that will be useful to evaluate future vaccine candidates.
AB - Background: In the present study, we applied microarray technology to define biosignatures by microarray transcriptome analysis in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, these biosignatures should be detectable without in vitro antigenic challenge. Principal Findings: After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytokine profile was found that correlated with vaccine-induced protective immunity following infection with virulent M. bovis in the lung as well as additional genes that were up-regulated in the spleens of vaccinated animals post-infection related to neutrophil biology and inflammation. Conclusions: This study has therefore prioritized both biomarkers predicting vaccination success before challenge and bio-signatures that are potentially associated with protective immune responses that will be useful to evaluate future vaccine candidates.
UR - http://www.scopus.com/inward/record.url?scp=77956220982&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0011319
DO - 10.1371/journal.pone.0011319
M3 - Article
C2 - 20596522
AN - SCOPUS:77956220982
SN - 1932-6203
VL - 5
JO - PLoS One
JF - PLoS One
IS - 6
M1 - e11319
ER -
