New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)

I. Pasakinskiene; K. Anamthawat-Jonsson; M. W. Humphreys; V. Paplauskiene; Robert Neil Jones

doi:10.1046/j.1365-2540.1998.00446.x

New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)

I. Pasakinskiene, K. Anamthawat-Jonsson, M. W. Humphreys, V. Paplauskiene, Robert Neil Jones

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The level of similarity between the DNA of Lolium multiflorum and three Festuca species, F. arundinacea, F. pratensis and F. glaucescens, was analysed on Southern blots, using DNA–DNA hybridization, and also on chromosomes using genomic in situ hybridization (GISH). It was demonstrated that the close relationship between L. multiflorum and the allohexaploid F. arundinacea arises principally from the affinity of L. multiflorum to one of the ancestral progenitors of F. arundinacea, namely F. pratensis. Using probes made from total genomic DNA of L. multiflorum, and also of L. perenne, particular regions of high homology, described here as ‘GISH bands’ were identified on F. pratensis chromosomes. These GISH bands, in combination with a specific probe for rDNA, provide us with new markers for the identification of chromosomes within the F. pratensis complement. Restriction analysis within the rDNA repeat unit revealed additional information on the close phylogeny of L. multiflorum and F. pratensis. The rDNA restriction patterns confirm that F. arundinacea originated as a hybrid between F. pratensis and F. glaucescens.

Original language	English
Pages (from-to)	659-665
Number of pages	7
Journal	Heredity
Volume	81
Issue number	6
DOIs	https://doi.org/10.1046/j.1365-2540.1998.00446.x https://doi.org/10.1038/sj.hdy.6884460
Publication status	Published - 1998

Keywords

GISH bands
Lolium/Festuca
Phylogeny
Restriction analysis
rDNA

Access to Document

10.1046/j.1365-2540.1998.00446.xLicence: Unclear 1
10.1038/sj.hdy.6884460Licence: Unclear 1

Permanent link

Persistent link

Cite this

@article{8285b08364bf40449fb9129be3f2ae62,

title = "New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)",

abstract = "The level of similarity between the DNA of Lolium multiflorum and three Festuca species, F. arundinacea, F. pratensis and F. glaucescens, was analysed on Southern blots, using DNA–DNA hybridization, and also on chromosomes using genomic in situ hybridization (GISH). It was demonstrated that the close relationship between L. multiflorum and the allohexaploid F. arundinacea arises principally from the affinity of L. multiflorum to one of the ancestral progenitors of F. arundinacea, namely F. pratensis. Using probes made from total genomic DNA of L. multiflorum, and also of L. perenne, particular regions of high homology, described here as {\textquoteleft}GISH bands{\textquoteright} were identified on F. pratensis chromosomes. These GISH bands, in combination with a specific probe for rDNA, provide us with new markers for the identification of chromosomes within the F. pratensis complement. Restriction analysis within the rDNA repeat unit revealed additional information on the close phylogeny of L. multiflorum and F. pratensis. The rDNA restriction patterns confirm that F. arundinacea originated as a hybrid between F. pratensis and F. glaucescens.",

keywords = "GISH bands, Lolium/Festuca, Phylogeny, Restriction analysis, rDNA",

author = "I. Pasakinskiene and K. Anamthawat-Jonsson and Humphreys, {M. W.} and V. Paplauskiene and Jones, {Robert Neil}",

note = "Funding Information: This work was supported by a Royal Society/NATO fellowship and a fellowship provided by The Minis-try of Culture and Education in Iceland. I.P. wishes to thank Zina Adriulaitiene, John Harper and Ian Sant for their valuable technical assistance, and Zbigniew Zwierzykowski and Lukas Wolters for providing some seeds.",

year = "1998",

doi = "10.1046/j.1365-2540.1998.00446.x",

language = "English",

volume = "81",

pages = "659--665",

journal = "Heredity",

issn = "0018-067X",

publisher = "Springer Nature",

number = "6",

}

TY - JOUR

T1 - New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)

AU - Pasakinskiene, I.

AU - Anamthawat-Jonsson, K.

AU - Humphreys, M. W.

AU - Paplauskiene, V.

AU - Jones, Robert Neil

N1 - Funding Information: This work was supported by a Royal Society/NATO fellowship and a fellowship provided by The Minis-try of Culture and Education in Iceland. I.P. wishes to thank Zina Adriulaitiene, John Harper and Ian Sant for their valuable technical assistance, and Zbigniew Zwierzykowski and Lukas Wolters for providing some seeds.

PY - 1998

Y1 - 1998

N2 - The level of similarity between the DNA of Lolium multiflorum and three Festuca species, F. arundinacea, F. pratensis and F. glaucescens, was analysed on Southern blots, using DNA–DNA hybridization, and also on chromosomes using genomic in situ hybridization (GISH). It was demonstrated that the close relationship between L. multiflorum and the allohexaploid F. arundinacea arises principally from the affinity of L. multiflorum to one of the ancestral progenitors of F. arundinacea, namely F. pratensis. Using probes made from total genomic DNA of L. multiflorum, and also of L. perenne, particular regions of high homology, described here as ‘GISH bands’ were identified on F. pratensis chromosomes. These GISH bands, in combination with a specific probe for rDNA, provide us with new markers for the identification of chromosomes within the F. pratensis complement. Restriction analysis within the rDNA repeat unit revealed additional information on the close phylogeny of L. multiflorum and F. pratensis. The rDNA restriction patterns confirm that F. arundinacea originated as a hybrid between F. pratensis and F. glaucescens.

AB - The level of similarity between the DNA of Lolium multiflorum and three Festuca species, F. arundinacea, F. pratensis and F. glaucescens, was analysed on Southern blots, using DNA–DNA hybridization, and also on chromosomes using genomic in situ hybridization (GISH). It was demonstrated that the close relationship between L. multiflorum and the allohexaploid F. arundinacea arises principally from the affinity of L. multiflorum to one of the ancestral progenitors of F. arundinacea, namely F. pratensis. Using probes made from total genomic DNA of L. multiflorum, and also of L. perenne, particular regions of high homology, described here as ‘GISH bands’ were identified on F. pratensis chromosomes. These GISH bands, in combination with a specific probe for rDNA, provide us with new markers for the identification of chromosomes within the F. pratensis complement. Restriction analysis within the rDNA repeat unit revealed additional information on the close phylogeny of L. multiflorum and F. pratensis. The rDNA restriction patterns confirm that F. arundinacea originated as a hybrid between F. pratensis and F. glaucescens.

KW - GISH bands

KW - Lolium/Festuca

KW - Phylogeny

KW - Restriction analysis

KW - rDNA

UR - http://hdl.handle.net/2160/36451

UR - http://www.scopus.com/inward/record.url?scp=0032421089&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2540.1998.00446.x

DO - 10.1046/j.1365-2540.1998.00446.x

M3 - Article

SN - 0018-067X

VL - 81

SP - 659

EP - 665

JO - Heredity

JF - Heredity

IS - 6

ER -

New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Correction to: New molecular evidence on genome relationships and chromosome identification in fescue (Festuca) and ryegrass (Lolium)

Cite this