Nitric oxide and hydrogen sulfide protect plasma membrane integrity and mitigate chromium-induced methylglyoxal toxicity in maize seedlings

Oussama Kharbech*, Lamia Sakouhi, Marouane Ben Massoud, Luis Alejandro Jose Mur, Francisco J. Corpas, Wahbi Djebali, Abdelilah Chaoui

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (SciVal)

Abstract

The present study aims to analyse the potential crosstalk between nitric oxide (NO) and hydrogen sulfide (H2S) in triggering resilience of maize (Zea mays L.) seedlings to hexavalent chromium (Cr VI). Exogenous application of 500 μM sodium nitroprusside (SNP, as a NO donor) or sodium hydrosulfide (NaHS, as a H2S donor) to 9-day-old maize seedlings, countered a Cr (200 μM) -elicited reduction in embryonic axis biomass. Cr caused cellular membrane injury by enhancing the levels of superoxide and hydroxyl radicals as well as methylglyoxal, and 4-hydroxy-2-nonenal. The application of SNP or NaHS considerably improved the endogenous NO and H2S pool, decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and improving some antioxidant enzymes activities in radicles and epicotyls. Radicles were more affected than epicotyls by Cr-stress with enhanced electrolyte leakage and decreased proton extrusion as indicated by lesser H+-ATPase activity. H2S appeared to mitigate Cr toxicity through up-regulated H+-ATPase and glyoxalase pathways and by maintaining optimal GSH levels as downstream effects of ROS and MG suppression. Hence, H2S-mediated the regeneration of GSH pool is associated with the attenuation of MG toxicity by enhancing S-lactoglutathione and D-lactate production. Taken together, our results indicate complementary roles for H2S and GSH to strengthen membrane integrity against Cr stress in maize seedlings.

Original languageEnglish
Pages (from-to)244-255
Number of pages12
JournalPlant Physiology and Biochemistry
Volume157
Early online date23 Oct 2020
DOIs
Publication statusPublished - 01 Dec 2020

Keywords

  • Chromium
  • Glutathione
  • Hydrogen sulfide
  • Membrane integrity
  • Methylglyoxal
  • Nitric oxide
  • Chromium/toxicity
  • Reactive Oxygen Species/metabolism
  • Seedlings/drug effects
  • Cell Membrane/drug effects
  • Proton-Translocating ATPases/metabolism
  • Glutathione/metabolism
  • Zea mays/drug effects
  • Pyruvaldehyde/toxicity
  • Hydrogen Sulfide/pharmacology
  • Nitric Oxide/pharmacology

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