Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids

I. Pasakinskiene; K. Anamthawat-Jonsson; M.W. Humphreys; R.N. Jones

doi:10.1038/hdy.1997.74

Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids

I. Pasakinskiene, K. Anamthawat-Jonsson, M.W. Humphreys, R.N. Jones

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Crosses were made between diploid Lolium multiflorum (2n = 2x = 14) and hexaploid Festuca arundinacea (2n = 6x = 42) in order to produce new forage hybrids. The F1 were treated with colchicine to double the chromosome number and to restore fertility in the amphidiploids (2n = 8x = 56). Chromosome counting in fertile hybrids revealed some unexpected diploid plants (2n = 14), as well as the expected amphiploids with 2n = 56. The phenotype of the diploids showed Festuca-like inflorescences which indicated they were not selfs of the L. multiflorum parent. Chromosome painting with a L. multiflorum genomic DNA probe showed the diploid complements to be recombinant and to carry some whole chromosomes, and some chromosome segments of L. multiflorum together with other chromosome parts which originated from the F. arundinacea genome. Meiotic behaviour of the diploids was regular, with seven bivalents. The results are discussed in terms of the segregation and recombination of different genomic components in the F1 hybrids during diploidization.

Original language	English
Pages (from-to)	464-469
Number of pages	6
Journal	Heredity
Volume	78
Issue number	5
DOIs	https://doi.org/10.1038/hdy.1997.74
Publication status	Published - May 1997

Keywords

chromosome elimination
Festuca arundinacea
forage grasses
genomic in situ hybridization (GISH)
Lolium multiflorum
somatic recombination

Access to Document

10.1038/hdy.1997.74

Permanent link

Persistent link

Cite this

@article{7e6175ede74c442cbd885dac9ef29a54,

title = "Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids",

abstract = "Crosses were made between diploid Lolium multiflorum (2n = 2x = 14) and hexaploid Festuca arundinacea (2n = 6x = 42) in order to produce new forage hybrids. The F1 were treated with colchicine to double the chromosome number and to restore fertility in the amphidiploids (2n = 8x = 56). Chromosome counting in fertile hybrids revealed some unexpected diploid plants (2n = 14), as well as the expected amphiploids with 2n = 56. The phenotype of the diploids showed Festuca-like inflorescences which indicated they were not selfs of the L. multiflorum parent. Chromosome painting with a L. multiflorum genomic DNA probe showed the diploid complements to be recombinant and to carry some whole chromosomes, and some chromosome segments of L. multiflorum together with other chromosome parts which originated from the F. arundinacea genome. Meiotic behaviour of the diploids was regular, with seven bivalents. The results are discussed in terms of the segregation and recombination of different genomic components in the F1 hybrids during diploidization.",

keywords = "chromosome elimination, Festuca arundinacea, forage grasses, genomic in situ hybridization (GISH), Lolium multiflorum, somatic recombination",

author = "I. Pasakinskiene and K. Anamthawat-Jonsson and M.W. Humphreys and R.N. Jones",

note = "Funding Information: Izolda Paakinskien wishes to thank the following: Professors D. Wilson, H. Thomas, R. Wilkins, Mr D. Baker and Mrs R. Baker for their help and support in making the first professional contacts in the UK in 1993; The Ministry of Culture and Education in Iceland for a Fellowship to work at The Agricultural Research Institute (RALA) in 1994 and 1996; and The British Council, and the IGER Fellowship Scheme, for Fellowships held at the Institute of Grassland and Environmental Research (IGER), at Aberystwyth, UK. in 1995.",

year = "1997",

month = may,

doi = "10.1038/hdy.1997.74",

language = "English",

volume = "78",

pages = "464--469",

journal = "Heredity",

issn = "0018-067X",

publisher = "Springer Nature",

number = "5",

}

TY - JOUR

T1 - Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids

AU - Pasakinskiene, I.

AU - Anamthawat-Jonsson, K.

AU - Humphreys, M.W.

AU - Jones, R.N.

N1 - Funding Information: Izolda Paakinskien wishes to thank the following: Professors D. Wilson, H. Thomas, R. Wilkins, Mr D. Baker and Mrs R. Baker for their help and support in making the first professional contacts in the UK in 1993; The Ministry of Culture and Education in Iceland for a Fellowship to work at The Agricultural Research Institute (RALA) in 1994 and 1996; and The British Council, and the IGER Fellowship Scheme, for Fellowships held at the Institute of Grassland and Environmental Research (IGER), at Aberystwyth, UK. in 1995.

PY - 1997/5

Y1 - 1997/5

N2 - Crosses were made between diploid Lolium multiflorum (2n = 2x = 14) and hexaploid Festuca arundinacea (2n = 6x = 42) in order to produce new forage hybrids. The F1 were treated with colchicine to double the chromosome number and to restore fertility in the amphidiploids (2n = 8x = 56). Chromosome counting in fertile hybrids revealed some unexpected diploid plants (2n = 14), as well as the expected amphiploids with 2n = 56. The phenotype of the diploids showed Festuca-like inflorescences which indicated they were not selfs of the L. multiflorum parent. Chromosome painting with a L. multiflorum genomic DNA probe showed the diploid complements to be recombinant and to carry some whole chromosomes, and some chromosome segments of L. multiflorum together with other chromosome parts which originated from the F. arundinacea genome. Meiotic behaviour of the diploids was regular, with seven bivalents. The results are discussed in terms of the segregation and recombination of different genomic components in the F1 hybrids during diploidization.

AB - Crosses were made between diploid Lolium multiflorum (2n = 2x = 14) and hexaploid Festuca arundinacea (2n = 6x = 42) in order to produce new forage hybrids. The F1 were treated with colchicine to double the chromosome number and to restore fertility in the amphidiploids (2n = 8x = 56). Chromosome counting in fertile hybrids revealed some unexpected diploid plants (2n = 14), as well as the expected amphiploids with 2n = 56. The phenotype of the diploids showed Festuca-like inflorescences which indicated they were not selfs of the L. multiflorum parent. Chromosome painting with a L. multiflorum genomic DNA probe showed the diploid complements to be recombinant and to carry some whole chromosomes, and some chromosome segments of L. multiflorum together with other chromosome parts which originated from the F. arundinacea genome. Meiotic behaviour of the diploids was regular, with seven bivalents. The results are discussed in terms of the segregation and recombination of different genomic components in the F1 hybrids during diploidization.

KW - chromosome elimination

KW - Festuca arundinacea

KW - forage grasses

KW - genomic in situ hybridization (GISH)

KW - Lolium multiflorum

KW - somatic recombination

UR - http://www.scopus.com/inward/record.url?scp=0030917526&partnerID=8YFLogxK

U2 - 10.1038/hdy.1997.74

DO - 10.1038/hdy.1997.74

M3 - Article

SN - 0018-067X

VL - 78

SP - 464

EP - 469

JO - Heredity

JF - Heredity

IS - 5

ER -

Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinacea hybrids

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this