OsTSD2-mediated cell wall modification affects ion homeostasis and salt tolerance

Chuanying Fang, Kang Li, Yangyang Wu, Dehong Wang, Junjie Zhou, Xiaoli Liu, Yufei Li, Cheng Jin, Xianqing Liu, Luis Mur, Jie Luo

Research output: Contribution to journalArticlepeer-review

18 Citations (SciVal)

Abstract

Salt stress is a major environmental threat to meeting the food demands of an increasing global population. The identification and exploitation of salt adaption mechanisms in plants are therefore vital for crop breeding. We here define the rice mutant (sstm1) whose salt sensitivity was unambiguously assigned to a single T-DNA insertion through segregational analysis following backcrossing to the wild type line. Insertion was within OsTSD2, which encoded a pectin methyltransferase. The sstm1 and allelic mutants, collectively known as tsd2, displayed higher content of Na + and lower level of K + in the shoot, which is likely to lead to reduced salt tolerance. Molecular analysis revealed reduced expression of the genes maintaining K + /Na + homeostasis in tsd2, including OsHKT1;5, OsSOS1, and OsKAT1. Furthermore, OsTSD2 influenced ion distribution between the hull and the rice seed, which could improve food safety with heavy metal pollution. Amino acid levels tended to be increased in tsd2 mutants, implicating a role of pectin in the regulation of metabolism. Taken together, we have demonstrated an important facet of salt tolerance, which implicated OsTSD2-mediated cell wall pectin modification as a key component that could be widely applied in crop science.

Original languageEnglish
Pages (from-to)1503-1512
Number of pages10
JournalPlant, Cell and Environment
Volume42
Issue number5
Early online date07 Dec 2018
DOIs
Publication statusPublished - 01 May 2019

Keywords

  • cell wall modification
  • pectin methylation
  • salt stress
  • ion homeostasis
  • rice
  • Pectins/metabolism
  • Genes, Plant
  • Oryza/genetics
  • Sodium/metabolism
  • Potassium/metabolism
  • Salt Tolerance/genetics
  • Homeostasis/genetics
  • Cell Wall/metabolism
  • Methyltransferases/genetics
  • Mutation
  • Seeds/metabolism

Fingerprint

Dive into the research topics of 'OsTSD2-mediated cell wall modification affects ion homeostasis and salt tolerance'. Together they form a unique fingerprint.

Cite this