Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polymorphic DNA profiles

John J. Dooley, Stephen P. Harrison, Lance Mytton, Malcolm Dye, Ann Cresswell, Leif Skot, John R. Beeching

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Through the use of a single, random 15mer as a primer, between 1 and 12 DNA amplification products were obtained per strain from a selection of 84 Rhizobium and Bradyrhizobium isolates. A principal-coordinate analysis was used to analyse the resulting amplified DNA profiles and it was possible to assign isolates to specific groupings. Within the species Rhizobium leguminosarum, the biovar phaseoli formed a distinct group from the other biovars of the species, viciae and trifolii, which grouped together. Isolates of Rhizobium meliloti and Bradyrhizobium species formed their own clear, specific groups. Although it was possible to identify individual isolates on the basis of differences in their amplified DNA profiles, there was evidence that some amplified segments were conserved among individuals at the biovar and species levels.

Original languageEnglish
Pages (from-to)665-673
Number of pages9
JournalCanadian Journal of Microbiology
Volume39
Issue number7
DOIs
Publication statusPublished - Jul 1993

Keywords

  • DNA amplification
  • Rhizobium
  • random primers

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