Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

H. M. Vordermeier; W. G. Bessler

doi:10.1016/S0171-2985(87)80033-5

Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

H. M. Vordermeier, W. G. Bessler

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

Abstract

Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by ³H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

Original language	English
Pages (from-to)	245-251
Number of pages	7
Journal	Immunobiology
Volume	175
Issue number	3
DOIs	https://doi.org/10.1016/S0171-2985(87)80033-5
Publication status	Published - 01 Sept 1987

Keywords

Con A
concanavalin A
ELISA
enzyme-linked immunosorbent assay
lipopolysaccharide
LPS
SDS
sodium dodecyl sulphate
Lymphocyte Activation
Mice, Inbred Strains
Salmonella typhimurium
Porins
B-Lymphocytes/immunology
Bacterial Outer Membrane Proteins/pharmacology
Animals
Mice
In Vitro Techniques
Mitogens

Access to Document

10.1016/S0171-2985(87)80033-5

Permanent link

Persistent link

Cite this

@article{c5bce2be92334671bf56c27a89b50d3a,

title = "Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins",

abstract = "Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.",

keywords = "Con A, concanavalin A, ELISA, enzyme-linked immunosorbent assay, lipopolysaccharide, LPS, SDS, sodium dodecyl sulphate, Lymphocyte Activation, Mice, Inbred Strains, Salmonella typhimurium, Porins, B-Lymphocytes/immunology, Bacterial Outer Membrane Proteins/pharmacology, Animals, Mice, In Vitro Techniques, Mitogens",

author = "Vordermeier, {H. M.} and Bessler, {W. G.}",

year = "1987",

month = sep,

day = "1",

doi = "10.1016/S0171-2985(87)80033-5",

language = "English",

volume = "175",

pages = "245--251",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

AU - Vordermeier, H. M.

AU - Bessler, W. G.

PY - 1987/9/1

Y1 - 1987/9/1

N2 - Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

AB - Porins were prepared from the outer membrane of Salmonella typhimurium and consisted of three polypeptides with similar molecular ratios around 40 kDa, and isoelectric points around 4.7. They constituted potent polyclonal activators for murine B lymphocytes. The compounds were mitogenic towards splenic lymphocytes of lipopolysaccharide responder Balb/c, non-responder (C3H/Hej), and athymic (Balb/c nu/nu) mouse inbred strains, as measured by 3H-thymidine incorporation into DNA. Furthermore, porin-activated B cells differentiated into immunoglobulin-secreting cells, as was measured by an ELISA test. The porins will be valuable in elucidating the molecular mechanism of lymphocyte activation in bacterial infections.

KW - Con A

KW - concanavalin A

KW - ELISA

KW - enzyme-linked immunosorbent assay

KW - lipopolysaccharide

KW - LPS

KW - SDS

KW - sodium dodecyl sulphate

KW - Lymphocyte Activation

KW - Mice, Inbred Strains

KW - Salmonella typhimurium

KW - Porins

KW - B-Lymphocytes/immunology

KW - Bacterial Outer Membrane Proteins/pharmacology

KW - Animals

KW - Mice

KW - In Vitro Techniques

KW - Mitogens

UR - http://www.scopus.com/inward/record.url?scp=0023232968&partnerID=8YFLogxK

U2 - 10.1016/S0171-2985(87)80033-5

DO - 10.1016/S0171-2985(87)80033-5

M3 - Article

C2 - 2824346

AN - SCOPUS:0023232968

SN - 0171-2985

VL - 175

SP - 245

EP - 251

JO - Immunobiology

JF - Immunobiology

IS - 3

ER -

Polyclonal Activation of Murine B Lymphocytes in vitro by Salmonella typhimurium Porins

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this