Polymorphic nuclear microsatellite loci for studies of brown crab, Cancer pagurus L.

Niall J. Mckeown*, Paul W. Shaw

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (SciVal)

Abstract

Twelve polymorphic microsatellite DNA loci were isolated from the brown crab, Cancer pagurus L., by construction of microsatellite-enriched genomic libraries. Genotyping of 40 individuals from Norfolk (UK) revealed variable levels of locus polymorphism with an average of 7.75 alleles per locus (range 2-22). The observed and expected heterozygosities per locus ranged from 0.025 to 0.868 and from 0.025 to 0.947, respectively. No evidence of linkage disequilibrium was detected between pairs of loci and genotype proportions at all loci conformed to Hardy-Weinberg equilibrium expectations. The microsatellite loci developed constitute a suite of genetic markers applicable to numerous areas of C. pagurus research.

Original languageEnglish
Pages (from-to)653-655
Number of pages3
JournalMolecular Ecology Resources
Volume8
Issue number3
DOIs
Publication statusPublished - May 2008

Keywords

  • paternity
  • population structure
  • stock
  • Crustacea
  • brachyura
  • edible crab

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