Projects per year
Polyphenol oxidase (PPO) may have multiple functions in tissues depending on its cellular or tissue localization. Here we use PPO RNAi transformants of red clover (Trifolium pratense) to determine the role PPO plays in normal development of plants, and especially in N 2-fixing nodules. In red clover, PPO was not essential for either growth or nodule production, or for nodule function in plants grown under optimal, N-free conditions. However, absence of PPO resulted in a more reduced environment in all tissues, as measured by redox potential, and caused subtle developmental changes in nodules. Leaves and, to a lesser extent nodules, lacking PPO tended to accumulate phenolic compounds. A comparison of nodules of two representative contrasting clones by microscopy revealed that nodules lacking PPO were morphologically and anatomically subtly altered, and that phenolics accumulated in different cells and tissues. Developing nodules lacking PPO were longer, and there were more cell layers within the squashed cell layer (SCL), but the walls of these cells were less thickened and the cells were less squashed. Within the N 2-fixing zone, bacteroids appeared more granular and were less tightly packed together, and were similar to developmentally compromised bacteroids elicited by catalase mutant rhizobia reported elsewhere.
|Journal||Frontiers in Plant Science|
|Publication status||Published - 17 Dec 2014|
- RNai transformants
- Red clover
- polyphenol oxidase (PPO)
- antioxidant activity
- Raman microspectrometry
- RNAi transformants
- Antioxidant activity
- Polyphenol oxidase (PPO)
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Person: Teaching And Research
- 1 Finished
Post-harvest modifications and implications for recovery of bioenergy or biorenewables
Donnison, I., Shah, I. & Winters, A.
Biotechnology and Biological Sciences Research Council
01 Apr 2008 → 31 Mar 2012
Project: Externally funded research