Polysaccharide production in liquid cell suspension cultures of Phleum pratense L.

A. D. Hale, Christopher Pollock, Susan Dalton

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


The production of carbohydrates by cell suspension cultures of Phleum pratense (timothy grass) is described. Extracellular polysaccharides similar in monosaccharide composition to native cell wall polymers were accumulated, together with polymers of fructose (fructans). The fructans had similar properties to the intracellular reserve polymers found in intact plants, and were found in both cells and media of young, slow-growing cultures.
Production of extracellular polysaccharides differed in cultures grown on sucrose or equimolar glucose/fructose as carbon source. These differences were observed only when autoclaved media were used, and were not related to changes in either pH or osmolarity. Autoclaving medium containing radioactive glucose and fructose produced a novel, unidentified labelled compound which was absent in medium containing labelled sucrose.
Original languageEnglish
Pages (from-to)435-438
Number of pages4
JournalPlant Cell Reports
Issue number6
Publication statusPublished - Dec 1987


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