Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters, H. Martin Vordermeier, Shelley Rhodes, Bhagwati Khatri, Mitchell V. Palmer, Mayara F. Maggioli, Tyler C. Thacker, Jeffrey T. Nelson, Bruce V. Thomsen, Suelee Robbe-Austerman, Doris M. Bravo Garcia, Mark A. Schoenbaum, Mark S. Camacho, Jean S. Ray, Javan Esfandiari, Paul Lambotte, Rena Greenwald, Adrian Grandison, Alina Sikar-Gang, Konstantin P. Lyashchenko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (SciVal)
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Abstract

Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Original languageEnglish
Article number164
Number of pages7
JournalBMC Veterinary Research
Volume13
Issue number1
DOIs
Publication statusPublished - 07 Jun 2017

Keywords

  • Antibody
  • Bovine tuberculosis
  • Dual path platform
  • Multi-antigen print immunoassay
  • Mycobacterium bovis
  • Tuberculin skin test

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