Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters; H. Martin Vordermeier; Shelley Rhodes; Bhagwati Khatri; Mitchell V. Palmer; Mayara F. Maggioli; Tyler C. Thacker; Jeffrey T. Nelson; Bruce V. Thomsen; Suelee Robbe-Austerman; Doris M. Bravo Garcia; Mark A. Schoenbaum; Mark S. Camacho; Jean S. Ray; Javan Esfandiari; Paul Lambotte; Rena Greenwald; Adrian Grandison; Alina Sikar-Gang; Konstantin P. Lyashchenko

doi:10.1186/s12917-017-1085-5

Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters, H. Martin Vordermeier, Shelley Rhodes, Bhagwati Khatri, Mitchell V. Palmer, Mayara F. Maggioli, Tyler C. Thacker, Jeffrey T. Nelson, Bruce V. Thomsen, Suelee Robbe-Austerman, Doris M. Bravo Garcia, Mark A. Schoenbaum, Mark S. Camacho, Jean S. Ray, Javan Esfandiari, Paul Lambotte, Rena Greenwald, Adrian Grandison, Alina Sikar-Gang, Konstantin P. Lyashchenko^*

^*Corresponding author for this work

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP^®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Original language	English
Article number	164
Number of pages	7
Journal	BMC Veterinary Research
Volume	13
Issue number	1
DOIs	https://doi.org/10.1186/s12917-017-1085-5
Publication status	Published - 07 Jun 2017

Keywords

Antibody
Bovine tuberculosis
Dual path platform
Multi-antigen print immunoassay
Mycobacterium bovis
Tuberculin skin test

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Ray Waters, W., Martin Vordermeier, H., Rhodes, S., Khatri, B., Palmer, M. V., Maggioli, M. F., Thacker, T. C., Nelson, J. T., Thomsen, B. V., Robbe-Austerman, S., Bravo Garcia, D. M., Schoenbaum, M. A., Camacho, M. S., Ray, J. S., Esfandiari, J., Lambotte, P., Greenwald, R., Grandison, A., Sikar-Gang, A., & Lyashchenko, K. P. (2017). Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results. BMC Veterinary Research, 13(1), Article 164. https://doi.org/10.1186/s12917-017-1085-5

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title = "Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results",

abstract = "Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP{\textregistered}) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.",

keywords = "Antibody, Bovine tuberculosis, Dual path platform, Multi-antigen print immunoassay, Mycobacterium bovis, Tuberculin skin test",

author = "{Ray Waters}, W. and {Martin Vordermeier}, H. and Shelley Rhodes and Bhagwati Khatri and Palmer, {Mitchell V.} and Maggioli, {Mayara F.} and Thacker, {Tyler C.} and Nelson, {Jeffrey T.} and Thomsen, {Bruce V.} and Suelee Robbe-Austerman and {Bravo Garcia}, {Doris M.} and Schoenbaum, {Mark A.} and Camacho, {Mark S.} and Ray, {Jean S.} and Javan Esfandiari and Paul Lambotte and Rena Greenwald and Adrian Grandison and Alina Sikar-Gang and Lyashchenko, {Konstantin P.}",

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Ray Waters, W, Martin Vordermeier, H, Rhodes, S, Khatri, B, Palmer, MV, Maggioli, MF, Thacker, TC, Nelson, JT, Thomsen, BV, Robbe-Austerman, S, Bravo Garcia, DM, Schoenbaum, MA, Camacho, MS, Ray, JS, Esfandiari, J, Lambotte, P, Greenwald, R, Grandison, A, Sikar-Gang, A & Lyashchenko, KP 2017, 'Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results', BMC Veterinary Research, vol. 13, no. 1, 164. https://doi.org/10.1186/s12917-017-1085-5

TY - JOUR

T1 - Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

AU - Ray Waters, W.

AU - Martin Vordermeier, H.

AU - Rhodes, Shelley

AU - Khatri, Bhagwati

AU - Palmer, Mitchell V.

AU - Maggioli, Mayara F.

AU - Thacker, Tyler C.

AU - Nelson, Jeffrey T.

AU - Thomsen, Bruce V.

AU - Robbe-Austerman, Suelee

AU - Bravo Garcia, Doris M.

AU - Schoenbaum, Mark A.

AU - Camacho, Mark S.

AU - Ray, Jean S.

AU - Esfandiari, Javan

AU - Lambotte, Paul

AU - Greenwald, Rena

AU - Grandison, Adrian

AU - Sikar-Gang, Alina

AU - Lyashchenko, Konstantin P.

PY - 2017/6/7

Y1 - 2017/6/7

N2 - Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

AB - Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

KW - Antibody

KW - Bovine tuberculosis

KW - Dual path platform

KW - Multi-antigen print immunoassay

KW - Mycobacterium bovis

KW - Tuberculin skin test

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U2 - 10.1186/s12917-017-1085-5

DO - 10.1186/s12917-017-1085-5

M3 - Article

C2 - 28592322

AN - SCOPUS:85020200427

SN - 1746-6148

VL - 13

JO - BMC Veterinary Research

JF - BMC Veterinary Research

IS - 1

M1 - 164

ER -

Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Abstract

Keywords

UN SDGs

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