Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters; H. Martin Vordermeier; Shelley Rhodes; Bhagwati Khatri; Mitchell V. Palmer; Mayara F. Maggioli; Tyler C. Thacker; Jeffrey T. Nelson; Bruce V. Thomsen; Suelee Robbe-Austerman; Doris M. Bravo Garcia; Mark A. Schoenbaum; Mark S. Camacho; Jean S. Ray; Javan Esfandiari; Paul Lambotte; Rena Greenwald; Adrian Grandison; Alina Sikar-Gang; Konstantin P. Lyashchenko

doi:10.1186/s12917-017-1085-5

Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

W. Ray Waters
, H. Martin Vordermeier
, Shelley Rhodes
, Bhagwati Khatri
, Mitchell V. Palmer
, Mayara F. Maggioli
, Tyler C. Thacker
, Jeffrey T. Nelson
, Bruce V. Thomsen
, Suelee Robbe-Austerman
, Doris M. Bravo Garcia
, Mark A. Schoenbaum
, Mark S. Camacho
, Jean S. Ray
, Javan Esfandiari
, Paul Lambotte
, Rena Greenwald
, Adrian Grandison
, Alina Sikar-Gang
, Konstantin P. Lyashchenko^*

^*Corresponding author for this work

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

75 Downloads (Pure)

Abstract

Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP^®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

Original language	English
Article number	164
Number of pages	7
Journal	BMC Veterinary Research
Volume	13
Issue number	1
DOIs	https://doi.org/10.1186/s12917-017-1085-5
Publication status	Published - 07 Jun 2017

Keywords

Antibody
Bovine tuberculosis
Dual path platform
Multi-antigen print immunoassay
Mycobacterium bovis
Tuberculin skin test

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Ray Waters, W., Martin Vordermeier, H., Rhodes, S., Khatri, B., Palmer, M. V., Maggioli, M. F., Thacker, T. C., Nelson, J. T., Thomsen, B. V., Robbe-Austerman, S., Bravo Garcia, D. M., Schoenbaum, M. A., Camacho, M. S., Ray, J. S., Esfandiari, J., Lambotte, P., Greenwald, R., Grandison, A., Sikar-Gang, A., & Lyashchenko, K. P. (2017). Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results. BMC Veterinary Research, 13(1), Article 164. https://doi.org/10.1186/s12917-017-1085-5

@article{bf02d85924e64c60917f00a85eddacce,

title = "Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results",

abstract = "Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP{\textregistered}) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9\% (n = 92, US) to 96.0\% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.",

keywords = "Antibody, Bovine tuberculosis, Dual path platform, Multi-antigen print immunoassay, Mycobacterium bovis, Tuberculin skin test",

author = "\{Ray Waters\}, W. and \{Martin Vordermeier\}, H. and Shelley Rhodes and Bhagwati Khatri and Palmer, \{Mitchell V.\} and Maggioli, \{Mayara F.\} and Thacker, \{Tyler C.\} and Nelson, \{Jeffrey T.\} and Thomsen, \{Bruce V.\} and Suelee Robbe-Austerman and \{Bravo Garcia\}, \{Doris M.\} and Schoenbaum, \{Mark A.\} and Camacho, \{Mark S.\} and Ray, \{Jean S.\} and Javan Esfandiari and Paul Lambotte and Rena Greenwald and Adrian Grandison and Alina Sikar-Gang and Lyashchenko, \{Konstantin P.\}",

year = "2017",

month = jun,

day = "7",

doi = "10.1186/s12917-017-1085-5",

language = "English",

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Ray Waters, W, Martin Vordermeier, H, Rhodes, S, Khatri, B, Palmer, MV, Maggioli, MF, Thacker, TC, Nelson, JT, Thomsen, BV, Robbe-Austerman, S, Bravo Garcia, DM, Schoenbaum, MA, Camacho, MS, Ray, JS, Esfandiari, J, Lambotte, P, Greenwald, R, Grandison, A, Sikar-Gang, A & Lyashchenko, KP 2017, 'Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results', BMC Veterinary Research, vol. 13, no. 1, 164. https://doi.org/10.1186/s12917-017-1085-5

TY - JOUR

T1 - Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

AU - Ray Waters, W.

AU - Martin Vordermeier, H.

AU - Rhodes, Shelley

AU - Khatri, Bhagwati

AU - Palmer, Mitchell V.

AU - Maggioli, Mayara F.

AU - Thacker, Tyler C.

AU - Nelson, Jeffrey T.

AU - Thomsen, Bruce V.

AU - Robbe-Austerman, Suelee

AU - Bravo Garcia, Doris M.

AU - Schoenbaum, Mark A.

AU - Camacho, Mark S.

AU - Ray, Jean S.

AU - Esfandiari, Javan

AU - Lambotte, Paul

AU - Greenwald, Rena

AU - Grandison, Adrian

AU - Sikar-Gang, Alina

AU - Lyashchenko, Konstantin P.

PY - 2017/6/7

Y1 - 2017/6/7

N2 - Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

AB - Background: Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB. Conclusions: Thus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.

KW - Antibody

KW - Bovine tuberculosis

KW - Dual path platform

KW - Multi-antigen print immunoassay

KW - Mycobacterium bovis

KW - Tuberculin skin test

UR - https://www.scopus.com/pages/publications/85020200427

U2 - 10.1186/s12917-017-1085-5

DO - 10.1186/s12917-017-1085-5

M3 - Article

C2 - 28592322

AN - SCOPUS:85020200427

SN - 1746-6148

VL - 13

JO - BMC Veterinary Research

JF - BMC Veterinary Research

IS - 1

M1 - 164

ER -

Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Abstract

Keywords

UN SDGs

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