Predicting future biomass yield in Miscanthus using the carbohydrate metabolic profile as a biomarker

Anne Maddison, Anyela Camargo-Rodriguez, Ian Scott, Charlotte Jones, Dafydd Elias, Sarah Hawkins, Alice Massey, John Clifton-Brown, Niall McNamarra, Iain Donnison, Sarah Purdy

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Abstract

In perennial energy crop breeding programmes, it can take several years before a mature yield is reached when potential new varieties can be scored. Modern plant breeding technologies have focussed on molecular markers, but for many crop species, this technology is unavailable. Therefore, prematurity predictors of harvestable yield would accelerate the release of new varieties. Metabolic biomarkers are routinely used in medicine, but they have been largely overlooked as predictive tools in plant science. We aimed to identify biomarkers of productivity in the bioenergy crop, Miscanthus, that could be used prognostically to predict future yields. This study identified a metabolic profile reflecting productivity in Miscanthus by correlating the summer carbohydrate composition of multiple genotypes with final yield 6 months later. Consistent and strong, significant correlations were observed between carbohydrate metrics and biomass traits at two separate field sites over 2 years. Machine-learning feature selection was used to optimize carbohydrate metrics for support vector regression models, which were able to predict interyear biomass traits with a correlation (R) of >0.67 between predicted and actual values. To identify a causal basis for the relationships between the glycome profile and biomass, a 13C-labelling experiment compared carbohydrate partitioning between high- and low-yielding genotypes. A lower yielding and slower growing genotype partitioned a greater percentage of the 13C pulse into starch compared to a faster growing genotype where a greater percentage was located in the structural biomass. These results supported a link between plant performance and carbon flow through two rival pathways (starch vs. sucrose), with higher yielding plants exhibiting greater partitioning into structural biomass, via sucrose metabolism, rather than starch. Our results demonstrate that the plant metabolome can be used prognostically to anticipate future yields and this is a method that could be used to accelerate selection in perennial energy crop breeding programmes.

Original languageEnglish
Pages (from-to)1264-1278
Number of pages15
JournalGCB Bioenergy
Volume9
Issue number7
Early online date21 Jan 2017
DOIs
Publication statusPublished - 13 Jun 2017

Keywords

  • C
  • Miscanthus
  • bioenergy
  • biomarkers
  • carbohydrates
  • cell wall
  • soluble sugars
  • starch

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