Probing the role of cell wall feruloylation during maize development by differential expression of an apoplast targeted fungal ferulic acid esterase

Marcia M. de O Buanafina, M. Fernanda Buanafina, Sue Dalton, Phillip Morris, Marissa Kowalski, Manav K. Yadav, Lindsay Capper

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Abstract

While many aspects of the growth of maize are well understood, the role of cell wall feruloylation particularly during internode elongation has not been firmly established, but results so far indicate that it has significant implications for both biofuel feedstock conversion and for crop yield. The growth of the cell wall is achieved by synthesis, integration and cross-linking between wall polymers. As ferulate oxidative coupling of arabinoxylan side chains constitutes a significant type of cross-link in grass cell walls, it is expected to have a crucial role in plant growth. Making use of plants expressing an apoplast targeted Aspergillus niger FAEA under the control of either a constitutive or an inducible promoter, the role of cell wall feruloylation in maize internode expansion was investigated. Analysis of FAEA expressing plants showed that where FAEA was targeted to the apoplast under a constitutive promoter, plants varied in stature either from semi-dwarf plants with a 40-60% height reduction, to extreme dwarf mutants with over 90% reduction in plant heights compared to controls. Results indicate that disruption of cell wall feruloylation by FAEA occurs before the start of rapid internode expansion is initiated and affects the normal course of internode elongation, resulting in short internodes and dwarfed plants. In contrast, when under the inducible Lm See1 senescence promoter, FAEA activity was found to be low up to the VT stage of development but increased significantly at the VR stage as plants began to senesce, strongly suggesting that normal cell wall feruloylation is required for the process of internode expansion. In addition, with apoplast targeted expression of FAEA under control of the senescence enhanced promoter it was possible to demonstrate decreased cell wall feruloylation without affecting internode expansion or other aspects of plant development.

Original languageEnglish
Article numbere0240369
Number of pages29
JournalPLoS One
Volume15
Issue number10
DOIs
Publication statusPublished - 09 Oct 2020
Externally publishedYes

Keywords

  • Aspergillus niger/enzymology
  • Carboxylic Ester Hydrolases/genetics
  • Cell Wall/metabolism
  • Coumaric Acids/metabolism
  • Fungal Proteins/genetics
  • Plant Leaves/anatomy & histology
  • Plants, Genetically Modified/anatomy & histology
  • Promoter Regions, Genetic
  • Xylans/metabolism
  • Zea mays/anatomy & histology

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