Production of xylooligosaccharides from renewable agricultural lignocellulose biomass

Teresa Suárez Quiñones, Andy Retter, Philip J. Hobbs, Jörn Budde, Monika Heiermann, Matthias Plöchl, Rao Ravella

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21 Citations (SciVal)
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Efficient utilization of lignocellulosic biomass requires pretreatment in order to liberate cellulose from lignin and disrupt its recalcitrant crystalline structure before effective enzymatic hydrolysis can take place. Three different pretreatment methods (pressure cooking with dilute alkali and dilute acid as well as alkaline extraction) to recover the xylooligosaccharides fraction from five different grass silage samples, whole crop rye silage and maize silage were compared. The predominant end products released were xylobiose, xylotetraose, xylopentaose and xylohexaose whereas the xylooligosaccharides release pattern differed with the substrate. Maximum values of xylooligosaccharides was found for grass silage 17.26 g/L, whole crop rye silage 3.06 g/L and for maize silage 5.77 g/L. Results reveal the production of high value by-products from agricultural biomass. Advantages of the green-biorefinery concept include a resulting liquid fraction after pretreatment with very low contents of inhibitors such as furfural, hydroxymethylfurfural (HMF) and phenolic compounds. Document embargo 03/08/2016.
Original languageEnglish
Pages (from-to)147-155
Number of pages8
Issue number3-4
Early online date03 Aug 2015
Publication statusPublished - 2015


  • xylooligosaccharides
  • acidic hydrolysis
  • alkaline hydrolysis
  • enzyme saccharification
  • xylan extraction


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