Programmed cell death assays for plants

Alan M. Jones, Silvia Coimbra, Angelika Fath, Mariana Sottomayor, Howard Thomas

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Citations (SciVal)

Abstract

Programmed cell death (PCD) is a critical part of normal development at all stages of the plant life cycle. This chapter discusses protocols for PCD assays. DNA degradation is an important diagnostic factor in programmed cell death, and cleavage of DNA in internucleosomal fragments of 180 bp is considered a hallmark of apoptosis. The ability to isolate undegraded high molecular weight DNA from viable tissue is a requirement for studying PCD-induced DNA degradation because it ensures that any DNA degradation observed is not an artifact of the isolation procedure. The barley aleurone layer is a model system to study hormonally induced PCD in plants. In this tissue, gibberellic acid (GA) induces PCD, whereas abscisic acid (ABA) prevents this program. The chapter describes a method for DNA isolation from barley aleurone cells that minimizes DNA degradation during the purification process. It has been shown to work well for high molecular weight DNA analysis in a variety of other tissue and cell preparations, for example, Arabidopsis thaliana leaves.
Original languageEnglish
Title of host publicationApoptosis
Subtitle of host publicationCell Death
EditorsLawrence M. Schwartz, Jonathan D. Ashwell
PublisherElsevier
Pages437-451
Number of pages15
Volume66
ISBN (Print)9780125441650
DOIs
Publication statusPublished - 30 May 2001

Publication series

NameMethods in Cell Biology

Fingerprint

Dive into the research topics of 'Programmed cell death assays for plants'. Together they form a unique fingerprint.

Cite this