Promoter sequences for defining transgene expression

Huw D Jones, Caroline A. Sparks

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (SciVal)

Abstract

The design of reverse genetic experiments that utilize transgenic approaches often requires transgenes to be expressed in a predefined pattern and there is limited information regarding the gene expression profile for specific promoters. It is important that expression patterns are predetermined in the specific genotype targeted for transformation because the same promoter-transgene construct can produce different expression patterns in different host species. This chapter compares constitutive, targeted, or inducible promoters that have been characterized in specific cereal species.

Original languageEnglish
Title of host publicationTransgenic Wheat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter R. Shewry
Pages171-184
Number of pages14
Volume478
ISBN (Electronic)978-1-59745-379-0
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana press
Volume478
ISSN (Print)1064-3745

Keywords

  • Avena
  • Base Sequence
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Hordeum
  • Poaceae
  • Promoter Regions, Genetic
  • Transgenes
  • Triticum

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