TY - JOUR
T1 - Purification and characterisation of a novel cysteine conjugate β-lyase from the tapeworm Moniezia expansa
AU - Adcock, Harriet J.
AU - Brophy, Peter
AU - Teesdale-Spittle, Paul H.
AU - Buckberry, Lorraine D.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - The paper presents the first report of the purification of an invertebrate cysteine conjugate β-lyase (CCBL). CCBL activity was shown to predominate within the cytosolic fraction of tissue from the tapeworm Moniezia expansa. The monomeric cytosolic enzyme was isolated with a Mr of 72 kDa and co-purified with transaminase activity towards l-aspartate. The substrate profile for M. expansa CCBL is different from that of mammalian CCBLs. Exploiting the differences in mammalian and parasite substrate profiles will facilitate the development of helminth targeted conjugates which will not be activated by host (mammalian) CCBLs
AB - The paper presents the first report of the purification of an invertebrate cysteine conjugate β-lyase (CCBL). CCBL activity was shown to predominate within the cytosolic fraction of tissue from the tapeworm Moniezia expansa. The monomeric cytosolic enzyme was isolated with a Mr of 72 kDa and co-purified with transaminase activity towards l-aspartate. The substrate profile for M. expansa CCBL is different from that of mammalian CCBLs. Exploiting the differences in mammalian and parasite substrate profiles will facilitate the development of helminth targeted conjugates which will not be activated by host (mammalian) CCBLs
KW - Cysteine conjugate β-lyase
KW - Moniezia expansa
KW - Parasite
UR - http://www.scopus.com/inward/record.url?scp=0034656011&partnerID=8YFLogxK
U2 - 10.1016/S0020-7519(00)00024-2
DO - 10.1016/S0020-7519(00)00024-2
M3 - Article
SN - 0020-7519
VL - 30
SP - 567
EP - 571
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 5
ER -