Abstract
All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.
Original language | English |
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Pages (from-to) | 1251-1261 |
Number of pages | 11 |
Journal | Journal of Applied Microbiology |
Volume | 103 |
Issue number | 4 |
Early online date | 17 Apr 2007 |
DOIs | |
Publication status | Published - Oct 2007 |
Keywords
- Animal Nutritional Physiological Phenomena
- Animals
- Bacterial Typing Techniques
- Butyrivibrio
- Cattle
- Clostridium
- DNA, Bacterial
- Diet
- Female
- Gastrointestinal Contents
- Polymerase Chain Reaction
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Rumen
- Stearic Acids