Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach

D. Paillard; N. McKain; M. T. Rincon; K. J. Shingfield; D. I. Givens; R. J. Wallace

doi:10.1111/j.1365-2672.2007.03349.x

Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach

D. Paillard, N. McKain, M. T. Rincon, K. J. Shingfield, D. I. Givens, R. J. Wallace

Institute of Biological, Environmental, and Rural Sciences

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.

Original language	English
Pages (from-to)	1251-1261
Number of pages	11
Journal	Journal of Applied Microbiology
Volume	103
Issue number	4
Early online date	17 Apr 2007
DOIs	https://doi.org/10.1111/j.1365-2672.2007.03349.x
Publication status	Published - Oct 2007

Keywords

Animal Nutritional Physiological Phenomena
Animals
Bacterial Typing Techniques
Butyrivibrio
Cattle
Clostridium
DNA, Bacterial
Diet
Female
Gastrointestinal Contents
Polymerase Chain Reaction
RNA, Bacterial
RNA, Ribosomal, 16S
Rumen
Stearic Acids

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title = "Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach",

abstract = "All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.",

keywords = "Animal Nutritional Physiological Phenomena, Animals, Bacterial Typing Techniques, Butyrivibrio, Cattle, Clostridium, DNA, Bacterial, Diet, Female, Gastrointestinal Contents, Polymerase Chain Reaction, RNA, Bacterial, RNA, Ribosomal, 16S, Rumen, Stearic Acids",

author = "D. Paillard and N. McKain and Rincon, \{M. T.\} and Shingfield, \{K. J.\} and Givens, \{D. I.\} and Wallace, \{R. J.\}",

year = "2007",

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doi = "10.1111/j.1365-2672.2007.03349.x",

language = "English",

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journal = "Journal of Applied Microbiology",

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T1 - Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach

AU - Paillard, D.

AU - McKain, N.

AU - Rincon, M. T.

AU - Shingfield, K. J.

AU - Givens, D. I.

AU - Wallace, R. J.

PY - 2007/10

Y1 - 2007/10

N2 - All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.

AB - All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.

KW - Animal Nutritional Physiological Phenomena

KW - Animals

KW - Bacterial Typing Techniques

KW - Butyrivibrio

KW - Cattle

KW - Clostridium

KW - DNA, Bacterial

KW - Diet

KW - Female

KW - Gastrointestinal Contents

KW - Polymerase Chain Reaction

KW - RNA, Bacterial

KW - RNA, Ribosomal, 16S

KW - Rumen

KW - Stearic Acids

UR - http://hdl.handle.net/2160/12659

U2 - 10.1111/j.1365-2672.2007.03349.x

DO - 10.1111/j.1365-2672.2007.03349.x

M3 - Article

C2 - 17897229

SN - 1364-5072

VL - 103

SP - 1251

EP - 1261

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 4

ER -

Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach

Abstract

Keywords

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