Quantum Cascade Lasers-Based Detection of Nitric Oxide

Gracia Montilla-Bascón, Julien Mandon, Frans J. M. Harren, Luis A. J. Mur, Simona M. Cristescu, Elena Prats

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (SciVal)

Abstract

Despite the established importance of nitric oxide (NO) in many physiological and molecular processes in plants, most methods for quantifying NO are open to criticism This reflects the differing methods either lacking specificity or sensitivity, or even from an undue dependence of results on experimental conditions (i.e., chemical concentrations, pH, etc.). In this chapter we describe a protocol to measure gaseous NO produced by a biological sample using quantum cascade laser (QCL)-based spectroscopy. This technique is based on absorption of the laser light by the NO molecules which have been passed from a biological sample into an optical s cell that is equipped with two mirrors placed at both ends. This design greatly increases the interaction path length with the NO molecules due to multiple reflections of the light coupled inside the cell. Thus, the method is able to provide online, in planta measurements of the dynamics of NO production, being highly selective and sensitive (down to ppbv levels;1 ppbv = part per billion by volume mixing ratio = 1:10-9).

Original languageEnglish
Title of host publicationNitric Oxide
Subtitle of host publicationMethods and Protocols
EditorsAlexander Mengel, Christian Lindermayr
PublisherSpringer Nature
Pages49-57
Number of pages9
Volume1747
ISBN (Print)978-1-4939-7694-2, 149397694X
DOIs
Publication statusPublished - 12 May 2018

Publication series

NameMethods in Molecular Biology
PublisherSpringer Nature
ISSN (Print)1064-3745

Keywords

  • nitric oxide
  • quantum cascade laser
  • laser based infrared spectoscopy
  • multipass cell
  • drought stress

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