RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli

Massimiliano Zampini, Pauline Rees Stevens, Justin Pachebat, Alison Kingston-Smith, Luis Mur, Finbarr Hayes

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The ability to assemble DNA sequences de novo through efficient and powerful DNA fabrication methods is one of the foundational technologies of synthetic biology. Gene synthesis, in particular, has been considered the main driver for the emergence of this new scientific discipline. Here we describe RapGene, a rapid gene assembly technique which was successfully tested for the synthesis and cloning of both prokaryotic and eukaryotic genes through a ligation independent approach. The method developed in this study is a complete bacterial gene synthesis platform for the quick, accurate and cost effective fabrication and cloning of gene-length sequences that employ the widely used host Escherichia coli.

Original languageEnglish
Article number11302
JournalScientific Reports
Publication statusPublished - 11 Jun 2015


  • genetic engineering
  • applied microbiology
  • DNA recombination
  • metabolic engineering
  • Synthetic Biology/methods
  • Genes, Synthetic
  • DNA/chemical synthesis
  • Escherichia coli/genetics
  • Hydrozoa/genetics
  • Genes, Bacterial/genetics
  • GATA1 Transcription Factor/genetics
  • Animals
  • Polymerase Chain Reaction/methods
  • Spectinomycin/pharmacology
  • Genetic Engineering/methods
  • Drug Resistance, Bacterial/genetics
  • Green Fluorescent Proteins/genetics
  • Escherichia coli Proteins/genetics


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