TY - JOUR
T1 - Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene
AU - Regensbogenova, Martina
AU - Kisidayova, Svetlana
AU - Michalowski, Tadeusz
AU - Javorsky, Peter
AU - Moon-Van Der Staay, Seung Yeo
AU - Moon-Van Der Staay, Georg W. M.
AU - Hackstein, Johannes. H. P.
AU - McEwan, N. R.
AU - Jouany, Jean-Pierre
AU - Newbold, C. James
AU - Pristas, Peter
N1 - Regensbogenova, M., Kisidayova, S., Michalowski, T., Javorsky, P., Moon-Van Der Staay, S. Y., Moon-Van Der Staay, G. W. M., Hackstein, J. H. P., McEwan, N. R., Jouany, J. P., Newbold, C. J., Pristas, P. (2004). Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene. Acta Protozoologica, 43, 219-224.
Sponsorship: EU-ERCULE project (European Rumen Ciliate Collection QLRICT-2000-01455)
PY - 2004
Y1 - 2004
N2 - A rapid method has been developed for molecular identification of rumen ciliates without the need for cultivation. Total DNA
was isolated from single protozoal cells by the Chelex method and nearly complete protozoal 18S rRNA genes were amplified and subjected
to restriction fragment length polymorphism analysis. On the basis of restriction patterns generated a molecular key was elaborated allowing
identification of protozoa solely by a molecular technique without prior knowledge of morphology. No differences were observed between
identical species originating from different animals or geographic locations, or between morphological variants of the same species. The ARDREA analysis described here provides a rapid and convenient way for identification and diversity studies of rumen protozoa.
AB - A rapid method has been developed for molecular identification of rumen ciliates without the need for cultivation. Total DNA
was isolated from single protozoal cells by the Chelex method and nearly complete protozoal 18S rRNA genes were amplified and subjected
to restriction fragment length polymorphism analysis. On the basis of restriction patterns generated a molecular key was elaborated allowing
identification of protozoa solely by a molecular technique without prior knowledge of morphology. No differences were observed between
identical species originating from different animals or geographic locations, or between morphological variants of the same species. The ARDREA analysis described here provides a rapid and convenient way for identification and diversity studies of rumen protozoa.
M3 - Article
SN - 1689-0027
SP - 219
EP - 224
JO - Acta Protozoologica
JF - Acta Protozoologica
ER -