Regeneration of fertile transgenic tall fescue plants with a stable highly expressed foreign gene

B. Kuai, Susan Dalton, Andy J. E. Bettany, Phillip Morris

Research output: Contribution to journalArticlepeer-review

19 Citations (SciVal)

Abstract

One hundred and seventeen green tall fescue plants and 37 albino plants were regenerated from a glufosinate ammonium resistant callus clone co-transformed with the bar gene and the gusgene, both driven by the rice actin 1 promoter. The gus gene was not detectable in regenerated plants but the presence of the bar gene in these plants was detected by the polymerase chain reaction and integration of the bar gene into the genome by Southern blot hybridization. A high and stable expression of the bar gene was evident from the assay for phosphinothricin-N-acetyltransferase activity and from spraying plants with glufosinate ammonium herbicide. There was no detectable variation with respect to the level of bar gene expression among these plants. However, no inheritance of the bar gene was found in two populations of outcrossed progenies.
Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume58
Issue number2
DOIs
Publication statusPublished - Aug 1999

Keywords

  • Festuca arundinacea
  • Herbicide resistance
  • Phosphinothricin acetyltransferase gene
  • Transgene expression

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