Release of the extracellular matrix from conidia of Blumeria graminis in relation to germination

Akane Meguro, Keiko Fujita, Hitoshi Kunoh, Timothy L. W. Carver, Ralph L. Nicholson

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)

Abstract

The release of extracellular matrix (ECM) and the emergence of germ tubes from conidia ofBlumeria graminis were studied by light microscopy and micromanipulation. More prompt and frequent ECM release was confirmed on an artificial hydrophobic substratum than on an artificial hydrophilic substratum. Conidia initially incubated on the hydrophilic substratum were transferred by micromanipulation to either the hydrophobic or the hydrophilic substrata. Immediately after transfer onto the hydrophobic substratum, 75% of conidia released ECM, whereas only 16% did so upon transfer to the hydrophilic substratum. Conidia transferred onto the hydrophobic substratum produced a primary germ tube (PGT) more promptly and frequently than those transferred to the hydrophilic substratum. Thus, conidia recognize and respond to substratum hydrophobicity perhaps immediately after contact. When inoculated onto either isolated barley cuticle or the hydrophobic artificial substratum, 2/3 of the conidia produced a PGT from their polar regions. By contrast, on the hydrophilic substratum 2/3 of conidia did so from the side region. These results show that substratum hydrophobicity affects the location of PGT emergence from conidia. Furthermore, the study indicates that very rapid recognition of surface hydrophobicity by conidia promotes ECM release and this in turn may influence the location of PGT emergence.
Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalMycoscience
Volume42
Issue number2
DOIs
Publication statusPublished - Apr 2001

Keywords

  • Blumeria graminis
  • conidia contact
  • extracellular matrix
  • germination

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