TY - JOUR
T1 - Reproductive disturbances and phosphoglucoisomerase instability in festuca arundinacea (tall fescue) plants regenerated from callus and cell suspension cultures
AU - Garcia, A
AU - Dalton, Susan
AU - Humphreys, Mervyn Owen
N1 - Funding Information:
of IGER for kindly allowing the use of the laboratory equipment and for help and advice at every stage, Professor M.D. Hayward for allowing the use of the isozyme laboratory, and F. Potter for the statistical analyses. This work was carried out during the tenure of a postdoctoral grant from the Spanish CICYT.
PY - 1994/10
Y1 - 1994/10
N2 - Shoot tips of four Festuca arundinacea Schreb. genotypes (breeder's lines) were cultured in vitro in order to induce callus formation. In addition, cell suspension cultures were established from some of the calli. Plants were regenerated after at least 1 month under callus culture, and all of them remained hexaploid, except for one mixaploid. The flowering response was greatly altered, with some plants flowering without vernalization in the first year after culture, and one plant which did not flower even in the second year. Chromosome aberrations (reciprocal translocations, paracentric inversions, sticky chromosomes and deletions) were present in all genotypes, even after only 1 month in callus culture. There was probably an isochromosome in one plant regenerated from cell suspension culture. Abnormal microsporogenesis affecting pollen sterility was common and genotype dependent. Two genotypes showed instability at the phosphoglucoisomerase (PGI-2) isozyme locus.
AB - Shoot tips of four Festuca arundinacea Schreb. genotypes (breeder's lines) were cultured in vitro in order to induce callus formation. In addition, cell suspension cultures were established from some of the calli. Plants were regenerated after at least 1 month under callus culture, and all of them remained hexaploid, except for one mixaploid. The flowering response was greatly altered, with some plants flowering without vernalization in the first year after culture, and one plant which did not flower even in the second year. Chromosome aberrations (reciprocal translocations, paracentric inversions, sticky chromosomes and deletions) were present in all genotypes, even after only 1 month in callus culture. There was probably an isochromosome in one plant regenerated from cell suspension culture. Abnormal microsporogenesis affecting pollen sterility was common and genotype dependent. Two genotypes showed instability at the phosphoglucoisomerase (PGI-2) isozyme locus.
KW - Festuca arundinacea
KW - Isozymes
KW - Meiotic analysis
KW - Pollen sterility
KW - Somaclonal variation
UR - http://www.scopus.com/inward/record.url?scp=0028055668&partnerID=8YFLogxK
U2 - 10.1038/hdy.1994.182
DO - 10.1038/hdy.1994.182
M3 - Article
SN - 0018-067X
VL - 73
SP - 355
EP - 362
JO - Heredity
JF - Heredity
IS - 4
ER -