Restricted gene flow in fragmented populations of a wind-pollinated tree

Jim Provan*, Gemma Beatty, Andrea M. Hunter, Robbie A. McDonald, Emma McLaughlin, S. Jane Preston, Sian Wilson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (SciVal)

Abstract

Fragmentation of natural populations can have negative effects at the genetic level, thus threatening their evolutionary potential. Many of the negative genetic impacts of population fragmentation can be ameliorated by gene flow and it has been suggested that in wind-pollinated tree species, high or even increased levels of gene flow are a feature of fragmented populations, although several studies have disputed this. We have used a combination of nuclear microsatellites and allele-specific PCR (AS-PCR) analysis of chloroplast single nucleotide polymorphisms (SNPs) to examine the levels and patterns of genetic diversity and population differentiation in fragmented populations of juniper (Juniperus communis) in Ireland and inform conservation programs for the species. Significant population differentiation was found for both chloroplast and nuclear markers, indicating restricted gene flow, particularly over larger geographic scales. For conservation purposes, the existence of genetically distinct clusters and geographically localised chloroplast haplotypes suggests that the concept of provenance should be taken into account when formulating augmentation or reintroduction strategies. Furthermore, the potential lack of seed dispersal and seedling establishment means that ex-situ approaches to seed and seedling management may have to be considered.

Original languageEnglish
Pages (from-to)1521-1532
Number of pages12
JournalConservation Genetics
Volume9
Issue number6
Early online date28 Dec 2007
DOIs
Publication statusPublished - 28 Dec 2007

Keywords

  • Juniperus communis
  • Juniper
  • Habitat fragmentation
  • Gene flow
  • Phylogeography
  • JUNIPERUS-COMMUNIS L
  • PINUS-SYLVESTRIS L.
  • CHLOROPLAST DNA
  • MITOCHONDRIAL-DNA
  • PLANT-POPULATIONS
  • HABITAT FRAGMENTATION
  • FOREST FRAGMENTATION
  • MICROSATELLITE LOCI
  • NONCODING REGIONS
  • UNIVERSAL PRIMERS

Fingerprint

Dive into the research topics of 'Restricted gene flow in fragmented populations of a wind-pollinated tree'. Together they form a unique fingerprint.

Cite this