Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Huw D Jones, Angela Doherty, Huixia Wu

Research output: Contribution to journalArticlepeer-review

116 Citations (SciVal)
162 Downloads (Pure)

Abstract

Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

Original languageEnglish
Article number5
JournalPlant Methods
Volume1
Issue number1
DOIs
Publication statusPublished - 05 Sept 2005

Fingerprint

Dive into the research topics of 'Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat'. Together they form a unique fingerprint.

Cite this