Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Huw D Jones; Angela Doherty; Huixia Wu

doi:10.1186/1746-4811-1-5

Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Huw D Jones, Angela Doherty, Huixia Wu

Department of Life Sciences

Rothamsted Research

Research output: Contribution to journal › Article › peer-review

121 Citations (Scopus)

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Abstract

Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

Original language	English
Article number	5
Journal	Plant Methods
Volume	1
Issue number	1
DOIs	https://doi.org/10.1186/1746-4811-1-5
Publication status	Published - 05 Sept 2005

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AB - Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

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Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

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