Schistosoma mansoni venom allergen-like proteins: Phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity

Leonardo P. Farias, Iain W Chalmers, Samirah Perally, Henrique K. Rofatto, Colin J. Jackson, Martha Brown, Mariana I. Khouri, Mayra M. F. Barbosa, Paul J. Hensbergen, Cornelis H. Hokke, Luciana C. C. Leite, Karl F Hoffmann

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
173 Downloads (Pure)

Abstract

Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalInternational Journal for Parasitology
Volume49
Issue number8
Early online date25 May 2019
DOIs
Publication statusPublished - 01 Jul 2019

Keywords

  • venom allergen-like
  • immunological cross reactivity
  • tissue localisation
  • schistosoma mansoni
  • Schistosoma mansoni
  • Tissue localization
  • Venom allergen-like
  • Immunological cross reactivity
  • Antigens, Helminth/classification
  • Multigene Family
  • Dengue Vaccines/immunology
  • Electrophoresis, Polyacrylamide Gel
  • Helminth Proteins/classification
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Profiling
  • Phylogeny
  • Blotting, Western
  • Cross Reactions
  • Immune Sera/immunology
  • Animals
  • Mass Spectrometry
  • Schistosoma mansoni/chemistry
  • Transcription, Genetic
  • Allergens/classification

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