Selectable Markers: Antibiotic and Herbicide Resistance

Julia L Goodwin, Gabriela M. Pastori, Michael R. Davey, Huw D. Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (SciVal)

Abstract

The low efficiencies of most plant transformation methods necessitate the use of selectable marker genes to identify those cells that successfully integrate and express transferred DNA. Genes conferring resistance to various antibiotics or herbicides are commonly used in laboratory transformation research. They encode proteins that detoxify corresponding selection agents and allow the preferential growth of transformed cells. This chapter describes the application of two selection systems on the transformation of wheat. One is based on the nptII gene and corresponding aminoglycoside antibiotics, the other is based on the bar gene and corresponding glufosinate ammonium herbicides.

Original languageEnglish
Title of host publicationTransgenic Plants
Subtitle of host publicationMethods and Protocols
EditorsLeandro Peña
PublisherSpringer Nature
Pages191-201
Number of pages11
Volume286
ISBN (Electronic)978-1-59259-827-4
ISBN (Print)978-1-58829-263-6
DOIs
Publication statusPublished - 2004

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume286
ISSN (Print)1064-3745

Keywords

  • Base Sequence
  • Colorimetry
  • DNA Primers
  • Drug Resistance
  • Herbicides
  • Immunity, Innate
  • Plants, Genetically Modified
  • Plasmids
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Selection, Genetic
  • Transformation, Genetic
  • Triticum
  • Ubiquitin
  • Zea mays

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