@article{91efedcb48a84dadbc123e485b6cbaf3,
title = "Selection of candidate coding DNA barcoding regions for use on land plants",
abstract = "An in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multiple primers and a modified multiplexed tandem polymerase chain reaction yielded 85–94% amplification across taxa, and mean sequence differences between sister taxa of 6.1 from 156 bases of accD to 22 from 493 bases of matK. We conclude that loci should be combined for effective diagnosis, and recommend further investigation of the following six loci: matK, rpoB, rpoC1, ndhJ, ycf5 and accD.",
author = "Ford, {Caroline S.} and Ayres, {Karen L.} and Nicola Toomey and Nadia Haider and Stahl, {Jonathan Van Alphen} and Kelly, {Laura J.} and Niklas Wikstr{\"o}m and Hollingsworth, {Peter M.} and Duff, {R. Joel} and Hoot, {Sarah B.} and Cowan, {Robyn S.} and Chase, {Mark W.} and Wilkinson, {Michael J.}",
note = "Ford, C. S., Ayres, K. L., Toomey, N., Haider, N., Stahl, J. V., Kelly, L. J., Wikstrom, N., Hollingsworth, P. M., Duff, R. J., Hoot, S. B., Cowan, R. S., Chase, M. W., Wilkinson, M. J. (2009). Selection of candidate coding DNA barcoding regions for use on land plants. Botanical Journal of the Linnean Society, 159, (1), 1-11. Sponsorship: Alfred P. Sloan Foundation Gordon and Betty Moore Foundation IMPF: 00.98 RONO: 00 ",
year = "2009",
month = jan,
day = "15",
doi = "10.1111/j.1095-8339.2008.00938.x",
language = "English",
volume = "159",
pages = "1--11",
journal = "Botanical Journal of the Linnean Society",
issn = "1095-8339",
publisher = "Oxford University Press",
number = "1",
}