Selection of transformed plants

Huw D Jones, Caroline A. Sparks

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (SciVal)

Abstract

The low frequency and randomness of transgene integration into host cells, combined with the significant challenges of recovering whole plants from those rare events, makes the use of selectable marker genes routine in plant transformation experiments. For research applications that are unlikely to be grown in the field, strong herbicide- or antibiotic resistance is commonly used. Here we use genes conferring resistance to glufosinate herbicides as an example of a selectable marker in wheat transformation by either Agrobacterium or biolistics.

Original languageEnglish
Title of host publicationTransgenic Wheat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter. R. Shewry
PublisherSpringer Nature
Pages23-37
Number of pages15
Volume478
ISBN (Electronic)978-1-59745-379-0
ISBN (Print)978-1-58829-961-1
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume478
ISSN (Print)1064-3745

Keywords

  • Aminobutyrates
  • Avena
  • Biolistics
  • Drug Resistance
  • Genes, Plant
  • Genetic Engineering
  • Genetic Markers
  • Herbicides
  • Hordeum
  • Plant Development
  • Plants
  • Plants, Genetically Modified
  • Rhizobium
  • Triticum

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