Self-incompatibility requires GPI anchor remodeling by the poppy PGAP1 ortholog HLD1

Zongcheng Lin, Fei Xie, Marina Munoz Trivino, Tao Zhao, Frederik Coppens, Lieven Sterck, Maurice Bosch, Vernonica E. Franklin-Tong, Moritz Nowack

Research output: Contribution to journalArticlepeer-review

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Abstract

Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are tethered to the outer leaflet of the plasma membrane where they function as key regulators of a plethora of biological processes in eukaryotes. Self-incompatibility (SI) plays a pivotal role regulating fertilization in higher plants through recognition and rejection of “self” pollen. Here, we used Arabidopsis thaliana lines that were engineered to be self-incompatible by expression of Papaver rhoeas SI determinants for an SI suppressor screen. We identify HLD1/AtPGAP1, an ortholog of the human GPI-inositol deacylase PGAP1, as a critical component required for the SI response. Besides a delay in flowering time, no developmental defects were observed in HLD1/AtPGAP1 knockout plants, but SI was completely abolished. We demonstrate that HLD1/AtPGAP1 functions as a GPI-inositol deacylase and that this GPI-remodeling activity is essential for SI. Using GFP-SKU5 as a representative GPI-AP, we show that the HLD1/AtPGAP1 mutation does not affect GPI-AP production and targeting but affects their cleavage and release from membranes in vivo. Our data not only implicate GPI-APs in SI, providing new directions to investigate SI mechanisms, but also identify a key functional role for GPI-AP remodeling by inositol deacylation in planta.

Original languageEnglish
Pages (from-to)1909-1923.e5
JournalCurrent Biology
Volume32
Issue number9
DOIs
Publication statusPublished - 09 May 2022

Keywords

  • PROGRAMMED CELL-DEATH
  • GLYCOSYL-PHOSPHATIDYLINOSITOL
  • ARABINOGALACTAN-PROTEINS
  • ARABIDOPSIS-THALIANA
  • MEMBRANE-PROTEINS
  • FLOWERING PLANTS
  • PHOSPHOLIPASE-C
  • GROWTH
  • RECOGNITION
  • IDENTIFICATION
  • Arabidopsis/metabolism
  • Inositol/metabolism
  • Humans
  • Papaver/genetics
  • Glycosylphosphatidylinositols/genetics
  • Pollen/metabolism
  • self-incompatibility
  • HLD1
  • GPI-inositol deacylase
  • programmed cell death
  • Arabidopsis
  • pollen rejection
  • Papaver
  • AtPGAP1
  • GPI-AP remodeling

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