Projects per year
Abstract
Self-incompatibility (SI) is used by many angiosperms to prevent self-fertilization and inbreeding. In common poppy (Papaver rhoeas), interaction of cognate pollen and pistil S-determinants triggers programmed cell death (PCD) of incompatible pollen. We previously identified that reactive oxygen species (ROS) signal to SI-PCD. ROS-induced oxidative post-translational modifications (oxPTMs) can regulate protein structure and function. Here we have identified and mapped oxPTMs triggered by SI in incompatible pollen. Notably, SI-induced pollen had numerous irreversible oxidative modifications while untreated pollen had virtually none. Our data provide a valuable analysis of the protein targets of ROS in the context of SI-induction and comprise a benchmark because currently there are few reports of irreversible oxPTMs in plants. Strikingly, cytoskeletal proteins and enzymes involved in energy metabolism are a prominent target of ROS. Oxidative modifications to a phosphomimic form of a pyrophosphatase result in a reduction of its activity. Therefore, our results demonstrate irreversible oxidation of pollen proteins during SI and provide evidence that this modification can affect protein function. We suggest that this reduction in cellular activity could lead to PCD.
Original language | English |
---|---|
Pages (from-to) | 1391-1404 |
Number of pages | 14 |
Journal | Plant Physiology |
Volume | 183 |
Issue number | 3 |
Early online date | 22 Apr 2020 |
DOIs | |
Publication status | Published - 31 Jul 2020 |
Keywords
- Inorganic Pyrophosphatase/metabolism
- Amino Acid Sequence
- Pollen/drug effects
- Proteasome Endopeptidase Complex/metabolism
- Actins/metabolism
- Oxidation-Reduction
- Solubility
- Pollen Tube/drug effects
- Amino Acids/metabolism
- Nitrosation
- Papaver/drug effects
- Peptide Hydrolases/metabolism
- Cytoskeletal Proteins/metabolism
- Peptides/metabolism
- Self-Incompatibility in Flowering Plants/drug effects
- Protein Processing, Post-Translational/drug effects
- Hydrogen Peroxide/toxicity
- Plant Proteins/chemistry
Fingerprint
Dive into the research topics of 'Self-incompatibility triggers irreversible oxidative modification of proteins in incompatible pollen'. Together they form a unique fingerprint.Profiles
Projects
- 1 Finished
-
F-actin associated proteins implicate new mechanisms involved in SI-PCD
Bosch, M. (PI) & Franklin-Tong, V. E. (CoI)
Biotechnology and Biological Sciences Research Council
11 Sept 2017 → 10 Dec 2020
Project: Externally funded research