Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii

Alison J Woolford, R.glyn Hewinson, Martin Woodward, Jeremy W Dale

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.
Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 01 Mar 1997


  • bovine tuberculosis
  • MPB70 antigen
  • Mycobacterium kansasii


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