TY - JOUR
T1 - Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle
AU - Hayashi, Shinichiro
AU - Aso, Hisashi
AU - Watanabe, Shinichiro
AU - Nara, Hidetoshi
AU - Rose, Michael T.
AU - Ohwada, Shyuichi
AU - Yamaguchi, Takahiro
N1 - Hayashi, T., Aso, H., Watanabe, K., Nara, H., Rose, M. T., Ohwada, S., Yamaguchi, T. (2004). Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle Histochemistry and Cell Biology, 122, (5), 427-434.
Sponsorship: Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries (number 1523), Japan.
PY - 2004/11
Y1 - 2004/11
N2 - Various cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle.
AB - Various cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle.
KW - Bovine
KW - HGF
KW - IGF-I
KW - IGF-II
KW - Skeletal muscle
KW - Immunohistochemistry
KW - Humans
KW - Recombinant Proteins/pharmacology
KW - Cells, Cultured
KW - Hepatocyte Growth Factor/metabolism
KW - Muscle Fibers, Skeletal/drug effects
KW - Insulin-Like Growth Factor II/metabolism
KW - Muscle, Skeletal/cytology
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - RNA, Messenger/metabolism
KW - Insulin-Like Growth Factor I/metabolism
KW - Dose-Response Relationship, Drug
KW - Regeneration
KW - Myoblasts/drug effects
KW - Animals
KW - Time Factors
KW - Cattle
KW - Female
KW - Cell Proliferation/drug effects
UR - http://www.scopus.com/inward/record.url?scp=12944331555&partnerID=8YFLogxK
U2 - 10.1007/s00418-004-0704-y
DO - 10.1007/s00418-004-0704-y
M3 - Article
C2 - 15480739
SN - 0948-6143
VL - 122
SP - 427
EP - 434
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 5
ER -