Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Chris Pollock; Olga A. Koroleva; John F. Farrar; A. D. Tomos; Joe Gallagher

doi:10.1078/0176-1617-00285

Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Chris Pollock, Olga A. Koroleva, John F. Farrar, A. D. Tomos, Joe Gallagher

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

Original language	English
Pages (from-to)	1089-1092
Number of pages	4
Journal	Journal of Plant Physiology
Volume	158
Issue number	8
DOIs	https://doi.org/10.1078/0176-1617-00285
Publication status	Published - 2001

Keywords

Hordeum vulgare
Lolium temulentum
micro-sampling
single cell analysis

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10.1078/0176-1617-00285

http://hdl.handle.net/2160/3451

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@article{7142422917b94af5bfa533e6f1d5a69e,

title = "Single cell analysis technique for comparison of specific mRNA abundance in plant cells",

abstract = "A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.",

keywords = "Hordeum vulgare, Lolium temulentum, micro-sampling, single cell analysis",

author = "Chris Pollock and Koroleva, {Olga A.} and Farrar, {John F.} and Tomos, {A. D.} and Joe Gallagher",

note = "Gallagher, J. A., Koroleva, O. A., Tomos, A. D., Farrar, J. F., Pollock, C. J. (2001). Single cell analysis technique for comparison of specific mRNA abundance in plant cells. Journal of Plant Physiology, 158, (8), 1089-1092",

year = "2001",

doi = "10.1078/0176-1617-00285",

language = "English",

volume = "158",

pages = "1089--1092",

journal = "Journal of Plant Physiology",

issn = "0176-1617",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Single cell analysis technique for comparison of specific mRNA abundance in plant cells

AU - Pollock, Chris

AU - Koroleva, Olga A.

AU - Farrar, John F.

AU - Tomos, A. D.

AU - Gallagher, Joe

N1 - Gallagher, J. A., Koroleva, O. A., Tomos, A. D., Farrar, J. F., Pollock, C. J. (2001). Single cell analysis technique for comparison of specific mRNA abundance in plant cells. Journal of Plant Physiology, 158, (8), 1089-1092

PY - 2001

Y1 - 2001

N2 - A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

AB - A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

KW - Hordeum vulgare

KW - Lolium temulentum

KW - micro-sampling

KW - single cell analysis

U2 - 10.1078/0176-1617-00285

DO - 10.1078/0176-1617-00285

M3 - Article

SN - 0176-1617

VL - 158

SP - 1089

EP - 1092

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 8

ER -

Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Abstract

Keywords

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