Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Chris Pollock, Olga A. Koroleva, John F. Farrar, A. D. Tomos, Joe Gallagher

Research output: Contribution to journalArticlepeer-review

12 Citations (SciVal)

Abstract

A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.
Original languageEnglish
Pages (from-to)1089-1092
Number of pages4
JournalJournal of Plant Physiology
Volume158
Issue number8
DOIs
Publication statusPublished - 2001

Keywords

  • Hordeum vulgare
  • Lolium temulentum
  • Micro-sampling
  • Single cell analysis

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