Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Chris Pollock; Olga A. Koroleva; John F. Farrar; A. D. Tomos; Joe Gallagher

doi:10.1078/0176-1617-00285

Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Chris Pollock
, Olga A. Koroleva
, John F. Farrar
, A. D. Tomos
, Joe Gallagher

Institute of Biological, Environmental, and Rural Sciences

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

Original language	English
Pages (from-to)	1089-1092
Number of pages	4
Journal	Journal of Plant Physiology
Volume	158
Issue number	8
DOIs	https://doi.org/10.1078/0176-1617-00285
Publication status	Published - 2001

Keywords

Hordeum vulgare
Lolium temulentum
Micro-sampling
Single cell analysis

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10.1078/0176-1617-00285

http://hdl.handle.net/2160/3451

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@article{7142422917b94af5bfa533e6f1d5a69e,

title = "Single cell analysis technique for comparison of specific mRNA abundance in plant cells",

abstract = "A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.",

keywords = "Hordeum vulgare, Lolium temulentum, Micro-sampling, Single cell analysis",

author = "Chris Pollock and Koroleva, \{Olga A.\} and Farrar, \{John F.\} and Tomos, \{A. D.\} and Joe Gallagher",

note = "Gallagher, J. A., Koroleva, O. A., Tomos, A. D., Farrar, J. F., Pollock, C. J. (2001). Single cell analysis technique for comparison of specific mRNA abundance in plant cells. Journal of Plant Physiology, 158, (8), 1089-1092",

year = "2001",

doi = "10.1078/0176-1617-00285",

language = "English",

volume = "158",

pages = "1089--1092",

journal = "Journal of Plant Physiology",

issn = "1618-1328",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Single cell analysis technique for comparison of specific mRNA abundance in plant cells

AU - Pollock, Chris

AU - Koroleva, Olga A.

AU - Farrar, John F.

AU - Tomos, A. D.

AU - Gallagher, Joe

N1 - Gallagher, J. A., Koroleva, O. A., Tomos, A. D., Farrar, J. F., Pollock, C. J. (2001). Single cell analysis technique for comparison of specific mRNA abundance in plant cells. Journal of Plant Physiology, 158, (8), 1089-1092

PY - 2001

Y1 - 2001

N2 - A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

AB - A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

KW - Hordeum vulgare

KW - Lolium temulentum

KW - Micro-sampling

KW - Single cell analysis

UR - https://www.scopus.com/pages/publications/0034881725

U2 - 10.1078/0176-1617-00285

DO - 10.1078/0176-1617-00285

M3 - Article

SN - 1618-1328

VL - 158

SP - 1089

EP - 1092

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 8

ER -

Single cell analysis technique for comparison of specific mRNA abundance in plant cells

Abstract

Keywords

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