SNP Discovery and Chromosome Anchoring Provide the First Physically-Anchored Hexaploid Oat Map and Reveal Synteny with Model Species

Rebekah E. Oliver, Nicholas A. Tinker, Gerard R. Lazo, Shiaoman Chao, Eric N. Jellen, Martin L. Carson, Howard W. Rines, Donald E. Obert, Joseph D. Lutz, Irene Shackelford, Abraham B. Korol, Charlene P. Wight, Kyle M. Gardner, Jiro Hattori, Aaron D. Beattie, Åsmund Bjørnstad, J. Michael Bonman, Jean-luc Jannink, Mark E. Sorrells, Gina L. Brown-GuediraJennifer W. Mitchell Fetch, Stephen A. Harrison, Catherine J. Howarth, Amir Ibrahim, Frederic L. Kolb, Michael S. Mcmullen, J. Paul Murphy, Herbert W. Ohm, Brian G. Rossnagel, Weikai Yan, Kelci J. Miclaus, Jordan Hiller, Peter J. Maughan, Rachel R. Redman Hulse, Joseph M. Anderson, Emir Islamovic, Eric W. Jackson, Mingliang Xu (Editor)

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A physically anchored consensus map is foundational to modern genomics research; however, construction of such a map in oat (Avena sativa L., 2n = 6x = 42) has been hindered by the size and complexity of the genome, the scarcity of robust molecular markers, and the lack of aneuploid stocks. Resources developed in this study include a modified SNP discovery method for complex genomes, a diverse set of oat SNP markers, and a novel chromosome-deficient SNP anchoring strategy. These resources were applied to build the first complete, physically-anchored consensus map of hexaploid oat. Approximately 11,000 high-confidence in silico SNPs were discovered based on nine million inter-varietal sequence reads of genomic and cDNA origin. GoldenGate genotyping of 3,072 SNP assays yielded 1,311 robust markers, of which 985 were mapped in 390 recombinant-inbred lines from six bi-parental mapping populations ranging in size from 49 to 97 progeny. The consensus map included 985 SNPs and 68 previously-published markers, resolving 21 linkage groups with a total map distance of 1,838.8 cM. Consensus linkage groups were assigned to 21 chromosomes using SNP deletion analysis of chromosome-deficient monosomic hybrid stocks. Alignments with sequenced genomes of rice and Brachypodium provide evidence for extensive conservation of genomic regions, and renewed encouragement for orthology-based genomic discovery in this important hexaploid species. These results also provide a framework for high-resolution genetic analysis in oat, and a model for marker development and map construction in other species with complex genomes and limited resources.
Original languageEnglish
Article numbere58068
JournalPLoS One
Issue number3
Publication statusPublished - 22 Mar 2013


  • Avena/genetics
  • Chromosome Mapping/methods
  • Genome, Plant/genetics
  • Polymorphism, Single Nucleotide/genetics
  • Synteny/genetics


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