TY - JOUR
T1 - Specificity of CD8+ T cells from subunit-vaccinated and infected H-2b mice recognizing the 38 kDa antigen of Mycobacterium tuberculosis
AU - Zhu, Xiaojiu
AU - Stauss, Hans J.
AU - Ivanyi, Juraj
AU - Vordermeier, H. Martin
PY - 1997/12/1
Y1 - 1997/12/1
N2 - CD8+ T cells have been implicated in protective anti-tuberculous immune responses, but little is known about the identity of mycobacterial antigens recognized by CD8+ T cells. In this study we identified the Mycobacterium tuberculosis 38 kDa protein as a target for murine CD8+ cytotoxic T lymphocytes (CTL) which were induced by vaccination of C57BL/6 mice with DNA delivered with a plasmid, with transfected tumour cells or by infection with tubercle bacilli. Using overlapping synthetic peptides covering the whole protein sequence, peptides predicted to contain H-2Kb or H-2Db motifs, as well as naturally processed peptides, we were able to identify CTL epitopes. Differences were demonstrated in peptide specificity between CTL from immunized or M. tuberculosis-infected mice. The identified CTL epitopes could be important for future analysis of the involvement of CD8+ T cells in M. tuberculosis infections and for vaccine development.
AB - CD8+ T cells have been implicated in protective anti-tuberculous immune responses, but little is known about the identity of mycobacterial antigens recognized by CD8+ T cells. In this study we identified the Mycobacterium tuberculosis 38 kDa protein as a target for murine CD8+ cytotoxic T lymphocytes (CTL) which were induced by vaccination of C57BL/6 mice with DNA delivered with a plasmid, with transfected tumour cells or by infection with tubercle bacilli. Using overlapping synthetic peptides covering the whole protein sequence, peptides predicted to contain H-2Kb or H-2Db motifs, as well as naturally processed peptides, we were able to identify CTL epitopes. Differences were demonstrated in peptide specificity between CTL from immunized or M. tuberculosis-infected mice. The identified CTL epitopes could be important for future analysis of the involvement of CD8+ T cells in M. tuberculosis infections and for vaccine development.
KW - CD8
KW - Cytotoxic T lymphocyte
KW - Epitopes
KW - Mycobacterium tuberculosis
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=0030732236&partnerID=8YFLogxK
U2 - 10.1093/intimm/9.11.1669
DO - 10.1093/intimm/9.11.1669
M3 - Article
C2 - 9418128
AN - SCOPUS:0030732236
SN - 0953-8178
VL - 9
SP - 1669
EP - 1676
JO - International Immunology
JF - International Immunology
IS - 11
ER -