Splitting of a prevalent mycobacterium bovis spoligotype by variable- Number tandem-repeat typing reveals high heterogeneity in an evolving clonal group

Sabrina Rodriguez-Campos*, Yurena Navarro, Beatriz Romero, Lucía De Juan, Javier Bezos, Ana Mateos, Paul Golby, Noel H. Smith, Glyn R. Hewinson, Lucas DomÍnguez, Darío GarcÍa-De-Viedma, Alicia Aranaz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (SciVal)

Abstract

Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. This study applied mycobacterial interspersed repetitive-unit- variable-number tandem-repeat (MIRU-VNTR) typing to a random panel of 115 M. bovis isolates that are representative of the most frequent spoligotype in the Iberian Peninsula, SB0121. VNTR typing targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26 (VNTR2996), QUB11a (VNTR2163a), QUB11b (VNTR2163b), QUB26 (VNTR4052), and QUB3232 (VNTR3232). We found a high degree of diversity among the studied isolates (discriminatory index [D]-0.9856), which were split into 65 different MIRU-VNTR types. An alternative short-format MIRU-VNTR typing targeting only the four loci with the highest variability values was found to offer an equivalent discriminatory index. Minimum spanning trees using the MIRU-VNTR data showed the hypothetical evolution of an apparent clonal group. MIRU-VNTR analysis was also applied to the isolates of 176 animals from 15 farms infected by M. bovis SB0121; in 10 farms, the analysis revealed the coexistence of two to five different MIRU types differing in one to six loci, which highlights the frequency of undetected heterogeneity.

Original languageEnglish
Pages (from-to)3658-3665
Number of pages8
JournalJournal of Clinical Microbiology
Volume51
Issue number11
Early online date28 Aug 2013
DOIs
Publication statusPublished - 01 Nov 2013

Fingerprint

Dive into the research topics of 'Splitting of a prevalent mycobacterium bovis spoligotype by variable- Number tandem-repeat typing reveals high heterogeneity in an evolving clonal group'. Together they form a unique fingerprint.

Cite this